iPSC Expansion & Banking Service
Accelerate Your Stem Cell Research!
Are you currently facing challenges in maintaining a consistent supply of high-quality iPSCs, managing cell line variability, or struggling with the time-consuming and resource-intensive nature of iPSC culture? Creative Biolabs' iPSC Expansion and Banking Service helps you streamline your workflow and ensure a reliable source of validated iPSCs, enabling you to accelerate your research and development through our advanced cell culture expertise and robust quality control.
How Creative Biolabs' iPSC Expansion and Banking Service Can Assist Your Project?
Creative Biolabs' iPSC Expansion and Banking Service provides a comprehensive solution for researchers seeking to preserve and expand their valuable iPSC lines. We handle the complexities of cell culture, allowing you to focus on your core research objectives. Our service delivers a reliable supply of high-quality, well-characterized iPSCs, ready for your specific applications.
Our iPSC Expansion and Banking Service follows a well-defined and optimized workflow, ensuring the integrity and viability of your cell lines.
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Required Starting Materials: To initiate the service, clients typically provide:
- Existing iPSC lines (frozen vials).
- Detailed cell line documentation, including passage number, culture conditions, any relevant genetic modifications, and quality control data, such as mycoplasma testing results.
- Workflow:
Initial Consultation and Project Setup
We begin with a detailed consultation to understand your specific needs and project requirements. This includes assessing the provided materials, defining the expansion and banking strategy (master cell bank (MCB) and working cell bank (WCB) or WCB only), and your specific requirements.
Cell Line Receipt and Assay Set-up
Upon receiving your iPSC lines, we perform a thorough incoming QC check. This involves visual inspection for viability and contamination, and where necessary, additional testing to confirm cell identity, quality, and pathogen antigens.
Cell Expansion
Our expert technicians expand your iPSC lines under carefully controlled conditions, using optimized culture media and validated protocols (feeder-dependent, feeder-free, or transition conditions). This process ensures sufficient cell numbers for banking while maintaining the pluripotency and genetic stability of the cells.
iPSC Characterization
iPSC characterization service can be taken into consideration at this stage to further confirm the high quality of expanded iPSCs before cell cryopreservation.
Cryopreservation and Banking
Expanded cells are cryopreserved using a controlled-rate freezing method and stored in our secure, monitored cryogenic storage facility. Each batch is meticulously labeled and tracked to ensure traceability and easy retrieval.
Final QC and Release
Before releasing the banked iPSCs, we perform final QC testing, including viability assessment and mycoplasma testing.
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Final Deliverables:
- Cryopreserved iPSC vials, labeled with detailed information (cell line ID, passage number, date of cryopreservation).
- A comprehensive QC report, including data on cell viability, mycoplasma testing, and any other relevant characterization assays.
- A certificate of analysis (CoA) for each banked cell line.
- Estimated Timeframe: The typical timeframe for this service ranges from 4 to 8 weeks, depending on the initial cell line condition, expansion requirements, and the specific QC assays required.
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Why Choose Us?
Creative Biolabs is a trusted partner for iPSC expansion and banking, offering several key advantages:
Expertise
Our team comprises highly experienced scientists and technicians with extensive knowledge in iPSC culture, expansion, and cryopreservation.
Quality Assurance
We adhere to stringent quality control standards at every step of the process, ensuring the highest quality and consistency of your banked iPSCs.
Customization
We offer flexible service options to meet your specific needs, including customized expansion protocols, cell banking volumes, and characterization assays.
State-of-the-Art Facilities
Our advanced cell culture laboratories and cryogenic storage facilities are equipped with the latest technology to ensure optimal cell viability and stability.
Reliability
We provide secure and reliable cell banking services, minimizing the risk of cell loss due to equipment failure or contamination.
Case Study:
We create MCB and WCB for our in-house human iPSC lines after cell expansion. Each bank contains ≥ 50 vials of frozen stock with detailed labels. After thawing, the cells show typical iPSC morphology, strong stemness marker expression detected by IF staining (NANOG and OCT4) and FACS analysis (TRA-1-81 and SSEA-4), and stable and consistent proliferation for passages reflected by cell growth curves.
Fig.1 Example of our iPSC MCB and WCB strategy.
Customer Reviews:
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Introduction
Induced pluripotent stem cells (iPSCs) hold immense promise for regenerative medicine, disease modeling, and drug discovery. The ability to reprogram somatic cells into a pluripotent state provides a powerful tool for generating patient-specific cell lines and studying complex biological processes. The reliable expansion and banking of iPSCs are crucial for ensuring a consistent supply of high-quality cells for research and therapeutic applications. Creative Biolabs' iPSC Expansion and Banking Service provides a streamlined and efficient solution for researchers to overcome these challenges.
FAQs
Q: What types of iPSC lines can Creative Biolabs expand and bank?
A: We can expand and bank a wide range of iPSC lines, including those derived from various somatic cell sources. Contact us to discuss the specific requirements of your cell lines.
Q: How do you ensure the quality and stability of the expanded iPSCs?
A: Creative Biolabs adheres to stringent quality control protocols throughout the entire process, including regular testing for mycoplasma contamination, viability, and pluripotency marker expression detections. Our experienced team ensures the genetic stability of the cells during expansion.
Q: Can I customize the expansion and banking process to fit my specific research needs?
A: Yes, we offer flexible service options and can customize the expansion and banking process to meet your specific research requirements. Contact our team to discuss your project in detail.
Q: How do your services compare to expanding and banking iPSCs in-house?
A: Our services offer several advantages over in-house expansion and banking, including reduced risk of contamination, access to specialized expertise and equipment, and significant savings in time and resources. Let us handle the complexities, so you can focus on your research.
Related Services
In addition to our iPSC Expansion and Banking Service, Creative Biolabs offers a comprehensive portfolio of related services to support your stem cell research:
How to Contact Us
Creative Biolabs is committed to providing researchers with the highest quality iPSC expansion and banking services. Contact Our Team for More Information and to Discuss Your Project.
