GFP-encoding Oncolytic Herpes Simplex Virus 1 (ΔICP34.5, ΔUNG), CMV-GFP(Cat#: RepOV-0009XY)

This product is a GFP encoding oncolytic herpes simplex virus, which is based on HSV-1 with ICP34.5 and UNG deleted. ICP34.5 protein, is important for viral replication, viral exit from infected cells, prevention of the premature shut-off of protein synthesis in the infected host, and neurovirulence. Uracil DNA glycosylase (UNG, UDG) is the most abundant cellular enzyme encoded by the UL2 gene for removing uracil generated by hydrolytic deamination of cytosine or misincorporation of dUTP. The deletion of UDG may have contributed to lower rates of DNA repair during the passage or selection conditions. This product can be used in oncolytic virotherapy research and further recombinant HSV construction.

Specifications

Family Herpesviridae
Species Herpes simplex virus
Serotype Herpes simplex virus 1
Backbone HSV-1 (ΔICP34.5, ΔUNG)
Backbone Background Herpes simplex virus 1 and 2 (HSV-1 and HSV-2), also known as human herpesvirus 1 and 2 (HHV-1 and HHV-2), are two members of the human Herpesviridae family, a set of viruses that produce viral infections in the majority of humans. Modified Herpes simplex virus is considered as a potential therapy for cancer and has been extensively clinically tested to assess its oncolytic ability.
Gene Modification ΔICP34.5, ΔUNG
Promoter CMV
Transgene GFP
Type of Transgene Reporter gene
Related Target/Protein Green fluorescent protein
Capsid Modification None
Titer >1*10^8 PFU
Related Diseases Brain cancer

Transgene

Alternative Names GFP, gfp, green fluorescent protein
Gene ID 7011691
UniProt ID P42212

Information

Introduction The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxygen.

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