HepG2 In Vitro Capillary Chamber Cell Migration Assay

This assay is to provide HepG2-based In Vitro Capillary Chamber Cell Migration Assay to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

HepG2

Human

Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.

Hep G2

Hepatocellular Carcinoma

Oncology

In Vitro Capillary Chamber Cell Migration Assay

HepG2-cell based In Vitro Capillary Chamber Cell Migration Assay

Like the Boyden chamber assay, the capillary chamber migration assay also contains two separate chambers (one comprising of the cells and the other with a chemoattractant) connected with a small capillary. Cell migration and morphological responses in this assay can be observed by time-lapse microscopy. Automated real-time monitoring is also possible with this method and this assay is ideal for a rare type of cells and compounds, which are available in small quantities.

Cell Migration and Invasion Assays

The movement of cancer cells and formation of tumors in neighboring tissue is a sequential cascade known as invasion-metastatic cascade, which includes cancer cell invasion through extracellular matrix (ECM), intravasation into blood and colonization at sites after they exit from blood circulation. Migration and invasion are two different processes where, cell migration involves movement of cells without passing through a barrier such as ECM, whereas cell invasion involves passing through a barrier such as ECM while destroying it.

Cell Migration Assay

Cell Migration Assay