Reporter-encoding Oncolytic Vaccinia Virus Western Reserve (ΔE3L,ΔK3), pSL-(Dsred) (RepOV-0085WQ)
DatasheetThis product is a Dsred encoding oncolytic vaccinia virus, which is based on VACV-WR with E3L and K3L double deleted.Protein E3 plays a role in the inhibition of multiple cellular antiviral responses activated by dsRNA, such as inhibition of PKR activation, apoptosis, and IFN-mediated antiviral activities. Protein K3 acts as a pseudosubstrate for EIF2AK2/PKR kinase. Inhibits therefore eIF-2-alpha phosphorylation by host EIF2AK2/PKR kinase and prevents protein synthesis shutoff.The double deletion of E3L and K3L with oncolytic-rendered modifications could enhance an immune response to a poxvirus vaccine.This product can be used in oncolytic virotherapy research and vaccinie application.
| Specifications | |
|---|---|
| Family | Poxviridae |
| Species | Vaccinia virus |
| Serotype | Western Reserve |
| Backbone | VACV-WR(ΔE3L,ΔK3) |
| Backbone Background | VACV-WR strain derived from Wyeth through passaging in mice and shown high tumor selectivity and strong oncolytic effect in mouse models.The engineered VACV-WR could further enhance the immune activity and the efficacy of cancer therapies. |
| Gene Modification | ΔE3L,ΔK3 |
| Promoter | pSL |
| Transgene | Dsred |
| Type of Transgene | Reporter gene |
| Related Target/Protein | Discosoma red fluorescent protein |
| Capsid Modification | None |
| Titer | >1*10^8 PFU |
| Related Diseases | Breast cancer, Prostate cancer, Colon cancer, Cancer vaccine |
| Transgene | |
|---|---|
| Alternative Names | DsRed, Discosoma red fluorescent protein |
| Information | |
|---|---|
| Introduction | Red fluorescent protein (RFP) is a fluorophore that fluoresces red-orange when excited. Several variants have been developed using directed mutagenesis. The original was isolated from Discosoma, and named DsRed. Others are now available that fluoresce orange, red, and far-red.RFP is approximately 25.9 kDa. The excitation maximum is 558 nm, and the emission maximum is 583 nm. |
All services and products are for lab research only, not for any clinical use.
Other products
- Cytokine-expressing Oncolytic Vaccinia Virus Western Reserve (ΔK3L), pSE/L-(IL-21)
- Cytokine-expressing Oncolytic Vaccinia Virus Western Reserve (ΔK3L), pSE/L-(IL-24)
- Cytokine-expressing Oncolytic Vaccinia Virus Western Reserve (ΔK3L), pSE-mLIGHT
- Reporter-encoding Oncolytic Vaccinia Virus Western Reserve (ΔE3L,ΔK3), pSL-(GFP)
- Cytokine-expressing Oncolytic Vaccinia Virus Western Reserve (ΔK3L), pSL-mCCL2
- Reporter-encoding Oncolytic Vaccinia Virus Western Reserve (ΔE3L), p11k-(GFP)
- Cytokine-expressing Oncolytic Vaccinia Virus Western Reserve (ΔE3L), pSL-(IL-21)
- Cytokine-expressing Oncolytic Vaccinia Virus Western Reserve (ΔK3L), pSE-(IL-6)
