Creative Biolabs is a world-leading service provider of application-specific antibody development. Here, we introduce our in vitro diagnostic (IVD) antibody development and immunoassay development services targeting CRTh2 marker. Our state-of-the-art technologies ensure that our clients receive high-quality, stable, flexible, and cost-effective products and services.

Introduction of CRTh2

Chemoattractant Receptor-homologous molecule expressed on T-Helper type 2 cells (CRTH2), also named Prostaglandin D2 receptor 2 (DP2), is a human protein encoded by the PTGDR2 gene. It is a member of the class of prostaglandin receptors which bind with and respond to various prostaglandins. CRTH2 is a G-protein coupled receptor that binds to the ligand prostaglandin D2. CRTH2 is expressed on various cell types including T helper type 2 (Th2) cells, a subpopulation of CD8+ T cells, eosinophils, mast cells, innate lymphoid type 2 cells, and basophils in humans, but not on human Th1 cells.

CRTH2 activation can increase intracellular calcium levels and reduce intracellular cyclic adenosine monophosphate levels. Its activation triggers a diverse of signaling pathways including phospholipase C, phosphatidylinositol 3-kinase, and p38 mitogen-activated kinase. CRTH2 induces intracellular Ca2+ mobilization and chemotaxis in Th2 cells in a Gαi-dependent manner and mediates Prostaglandin D2-dependent cell migration of blood eosinophils and basophils. In vitro studies, as well as animal and human investigations showed that CRTH2 was mainly involved in immunomodulating effects, such as induction of migration, activation, and cytokine release of leukocytes, which is related to allergic, eosinophilic inflammation and asthma. Increasing studies discovered that CRTH2 might also impact on diseases of the central nervous system, kidney, intestine, lung, hair and skin, bone and cartilage, and even in cancer.

Scheme summarizing the proposed signal pathways used by PGD2/CRTH2 to activate human Th2 cells. Fig.1 Scheme summarizing the proposed signal pathways used by PGD2/CRTH2 to activate human Th2 cells. (Xue, 2007)

CRTh2 Marker of Sepsis

Sepsis is caused by an immune response triggered by an infection. Neutrophils play a critical role in the innate immune response of sepsis, including releasing cytokines, antimicrobial proteins, and phagocytizing invading microbes. Recent research by Ishii et al. (2012) indicated that CRTH2 knock-out mice performed highly resistant to cecal ligation and puncture (CLP)-induced sepsis. This resistance was mechanistically related to lower bacterial load, lower production of proinflammatory cytokines including tumor necrosis factor alpha, interleukin 6 and Chemokine (C-C motif) ligand 3 in peritoneal cavity and blood. And increased neutrophil accumulation based on up-regulation of the chemokine receptor CXCR2 also contributed to the CRTH2 knock-out resistance.

The function of DP and CRTH2 receptors in inflammation can be regarded as antagonistic. Fig.2 The function of DP and CRTH2 receptors in inflammation can be regarded as antagonistic. (Kupczyk, 2017)

Features

To aid in the prognosis and diagnosis of sepsis, Creative Biolabs offers an extensive range of IVD antibody development services against protein C antigens to measure its levels clinically. The features of our services include:

  • Expertise: expert in the development of antibodies for different immunoassays (e.g., ELISA, immunohistochemistry, Western blot, immunochromatography assay).
  • Experience: years of experience in the IVD field with the completion of numerous IVD-related projects.
  • Efficiency: fast turnaround time and competitive pricing.
  • Facility: fully equipped facility to perform antigen & antibody conjugation, protein characterization and purification.

Creative Biolabs provides IVD antibody development services against an extensive range of sepsis biomarkers in addition to CRTh2. During antibody development, we work hand in hand with you and your team to ensure project goals and timelines are being met. Contact us to discuss your projects and experience the great value of our expert services.

References

  1. Xue L Z, et al. (2007). “Inhibition of PI3K and calcineurin suppresses chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2)-dependent responses of Th2 lymphocytes to prostaglandin D2.” Biochemical Pharmacology. 73(6), 843-853.
  2. Ishii M, et al. (2012). “CRTH2 is a critical regulator of neutrophil migration and resistance to polymicrobial sepsis.” Journal of immunology. 188(11): 5655-5664.
  3. Kupczyk M., & Kuna P. (2017). “Targeting the PGD2/CRTH2/DP1 signaling pathway in asthma and allergic disease: current status and future perspectives.” Drugs, 77(12), 1281-1294.

For Research Use Only.


Related Services:

Online Inquiry
Name:
*Phone:
*E-mail Address:
*Service & Products Interested:
Project Description:
Contact Us USA

Tel:
Fax:
Email:
UK

Tel:
Email:

Germany

Tel:
Email:

Follow us on:
Copyright © 2024 Creative Biolabs.
Inquiry Basket