Kasumi-3 In Vitro NO Scavenging Activity Assay (Oxidative Stress)

This assay is to provide Kasumi-3-based In Vitro NO Scavenging Activity Assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

Kasumi-3

Human

The cell line was established from the blast cells of a myeloperoxidase-negative acute leukemia patient.
Treatment with either interleukin (IL)-2, IL-3, IL-4, granulocyte-macrophage colony-stimulating factor (GMCSF) or stem cell factor induced the proliferation, characteristic of undifferentiated leukemia.

Acute Myeloblastic Leukemia; AML

Oncology

In Vitro NO Scavenging Activity Assay (Oxidative Stress)

Kasumi-3-cell based In Vitro NO Scavenging Activity Assay (Oxidative Stress)

It's a commonly used simple antioxidant assay. The ability of antioxidants to scavenge NO. and hydroxyl free radicals (OH.) is measured in NO and hydrogen peroxide scavenging assays respectively.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Antioxidant Assay