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The anti-CMV pp65 (NLVPMVATV) TCR (clone TCRCD8-CMV#16) Jurkat cell line is a stable cell line made from the anti-CMV pp65 TCR lentivirus. The recombinant Jurkat T cell was designed to predict the MOA of CMV pp65 -TCR, measure the CMV pp65 -TCR specificity and screen target cells expressing CMV pp65. And the product can be used to treat Cytomegalovirus infection.
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Specifications
Cell Background
The Jurkat cell line was established from the peripheral blood of human T lymphocyte cells. Jurkat cells are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors. Jurkat cells can produce interleukin 2, and are used in research involving the susceptibility of cancers to drugs and radiation.
Cell Type
T lymphocyte
Formulation
Containing ≥ 1 X 10*6 / vial frozen cells
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Applications
• Screen for activators or inhibitors of CMV signaling in a cellular context • Characterize the biological activity of CMV and its interactions with ligands • Predict the MOA of the TCR design • Screen and validate CMV-expressing target cells
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). Once reconstituted, the cells may be used for up to five days if properly stored at 2°C - 8°C in the buffer provided.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance. Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line. Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
Our recombinant Jurkat cell are for research use only, not for diagnostic or therapeutic use.
Quality Control
Cultures are screened for the presence of bacteries, yeast, fungi and mycoplasma (DNA amplification). Growth media are also certified based on U.S. Public Health Service Guidelines.
Tumorgenicity
Positive (In vitro/vivo transformation assay)
Oncogenicity
Positive (In vitro soft agarose assay and life-time studies)
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus saftey assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
TCR Design
Target
CMV pp65
Introduction
Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period. CMV viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. pp65 is a 65kD phosphorylated glycoprotein and is the most abundant of the late antigens.
HLA
HLA-A0201
Common Name
CMV pp65
Target Species
CMV
TCR Clone
TCRCD8-CMV#16
TCR-Host Animal
Human
Vector Name
pCDTCR1
Vector length
~ 8 kb
Vector Type
Lentiviral vector
Targeting Diseases
Cytomegalovirus infection
Epitope
NLVPMVATV
Format
Non-modified TCR
Customer Reviews and Q&As
There are currently no customer reviews or questions for Anti-CMV pp65 (NLVPMVATV) T Cell Receptor (clone TCRCD8-CMV#16), Jurkat Cell Line (TCRJ-CQ352). Click the button below to contact us or submit your feedback about this product.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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