Creative Biolabs, a leader in the field of gene-engineered T cells for cancer therapy, offers gene packaging and delivery services for the generation of TCR-modified T cells. Relying on our advanced technologies and rich experience, we can provide the most professional TCR transfection for the most cutting-edge genetically modified immunotherapies.
T lymphocytes play a crucial role in immunity and there are various gene therapy strategies targeting T cells. The efficient gene transfer into T cells facilitates the treatment of several genetic dysfunctions, inherited diseases and acquired diseases. TCR gene transfer via cell fusion or electroporation is also shown to confer MHC restricted specificity to nonspecific T cells. However, these initial processes are generally inefficient for a great number of T cells for clinical application.
Retroviral: the advent of retroviral vectors not only makes gene transfer more efficient at large scale but also enables the stable integration of exogenous genes. Despite demonstrations of scalability and stable expression, retroviral vectors require full T-cell activation for efficient gene transduction that may, in certain culture systems, inhibit cell function.
Lentiviral: lentiviral vectors have the potential to be safer from the perspective of insertional mutagenesis and they have substantially higher transduction efficiency for genetically engineering human T cells. Recently, lentiviral vectors have emerged as a favorable vector system for stably expressing two-gene TCRs, which require dual gene expression. Bicistronic lentiviral vectors that provide for high-level tumor or viral antigen-specific TCR gene expression in T cells have been developed to show strong promise for clinical application.
Electroporation: electroporation-mediated mRNA transfection is an alternative and promising approach without many limitations associated with gene transfer vectors that insert in the genome. mRNA manufacture and delivery does not require the significant costs of manufacture and testing that exogenous viral vectors need.
Fig.1 Retroviral Gene Transfer to Generate Tumor-Specific T Cells
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