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Rhesus Macaque MHC Genotyping Service

Equipped with state-of-the-art facilities and experienced immunology experts, Creative Biolabs is dedicated to offering genotyping services for our global clients. With advanced technology platforms and extensive expertise, we are ready to provide you with high-quality rhesus macaque MHC genotyping services.

Background

The major histocompatibility complex (MHC) of rhesus macaque is a widely studied set of genes. Which plays a crucial role in experimental allografts of organs and stem cells, and influences rhesus macaque reproduction and numerous biological parameters. The genomic MHC region mainly contains clusters of genes that encode the MHC class I complex and the MHC class II complex. Studies show that the entire region from the MHC class I to the MHC class II of rhesus macaque (Mamu) is 4.72 Mb in length based on the genomic map data. Comparing to the classical HLA-A and HLA-B genes and the non-classical HLA-E, HLA-F, and HLA-G genes of human, the corresponding genes of rhesus macaque are identified in the α, β, κ block and designated Mamu-A, Mamu-B, Mamu-E, Mamu-F, and Mamu-G, respectively.

Genomic map of the protein coding MHC loci among the Mamu regions.Fig.1 Genomic map of the protein coding MHC loci among the Mamu regions. (Shiina T, 2016)

Rhesus Macaque MHC genotyping services

To identify the complexity of MHC polymorphism, Creative Biolabs developed targeted NGS technologies for some nonhuman species to genotype the polymorphisms of MHC genes. Targeted NGS method using DNA or RNA samples is a feasible, productive platform. Which can generate high-quality MHC allele information from a variety of samples unavailable by other genotyping methods. In addition, we developed a user-friendly computational application that interprets the raw data and reliably exports the final genotyping results in spreadsheet file format.

A schematic workflow of the successive steps of the MHC genotyping method by NGS amplicon sequencing for the rhesus macaque.Fig. 2 A schematic workflow of the successive steps of the MHC genotyping method by NGS amplicon sequencing for the rhesus macaque. (Shiina T, 2015)

Highlight Features

To provide end-to-end rhesus macaque MHC genotyping service, our team of highly trained immunologists and technicians will help you work out the best service for you, and manage the whole process from sample handling to final report delivery within several days.

Quality-assured:

MHC genotyping services are performed under strict quality systems. We offer accurate, clearly presented, easy to interpret reports.

Flexible sample types choices:

We can work with DNA or RNA samples.

Equipped with state-of-the-art technology platforms and extensive expertise, Creative Biolabs provides one-stop integrated services to help our worldwide clients boost their diverse research and projects. For further details about MHC genotyping services, please don't hesitate to contact us and see how we can help you reach your clinical vision.

References

  1. Shiina T, et al. Comparative genomics of the human, macaque and mouse major histocompatibility complex. John Wiley & Sons Ltd, Immunology. 2016, 150, 127-138.
  2. SShiina T, Blancher A. The Cynomolgus Macaque MHC Polymorphism in Experimental Medicine. Cells. 2019, 8, 978.
  3. SCaskey J R, et al. MHC genotyping from rhesus macaque exome sequences. Immunogenetics. 2019, 71(8-9): 531-544.
  4. SShiina T, et al. MHC Genotyping in Human and Nonhuman Species by PCR-based Next-Generation Sequencing. Next Generation Sequencing-Advances, Applications and Challenges. 2015

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

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