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pCDCAR1 GPC3 h(8ζ), T(CAR-T-2-L342-8Z)

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The vector of anti-GPC3 chimeric antigen receptor (CAR) is constructed for the engineering of T cells to target human GPC3. The T cells are genetically modified through transduction with a lentiviral vector expressing scFv of anti-GPC3 antibody linked to a CD8 hinge-transmembrane domain and CD3-zeta signaling domains. And the vector product was designed for the treatment of hepatocellular carcinoma (HCC).

Details

Target
Glypican-3 (GPC3)
Target Species
Human
Targeting Cell Type
T cell
Targeting Diseases
Hepatocellular Carcinoma (HCC)
Generation
Second
Vector Name
pCDCAR1
Vector Length
8kb
Vector Type
Lentiviral
Receptor Construction
scFv-CD8-CD3ζ
Discription of Signaling Cassetes
CD8 CD8 (cluster of differentiation 8) is a transmembrane glycoprotein that serves as a co-receptor for the T cell receptor(TCR). Like the TCR, CD8 binds to a major histocompatibility complex (MHC) molecule, but is specific for the class I MHC protein. This affinity between CD8 and MHC molecule keeps the chimeric T cell receptor and the target cell bound closely together during antigen-specific activation.
CD3ζ
CD3ζ, also known as T-cell receptor zeta, which together with T-cell receptor and CD3γ, δ , ε chain, forms the TCR-CD3 complex. ζ was expressed independently from the complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3-zeta,which contains 3 ITAMs, is the most commonly used endodomain component of CARs. It transmits an activation signal to the T cell after antigen is bound. CD3-zeta may not provide a fully competent activation signal and additional co-stimulatory signaling is needed. For example, chimeric CD28 and OX40 can be used with CD3-zeta to transmit a proliferative/survival signal, or all three can be used together.
Antibody Clone
9C2
Antibody Host
Mouse

Target

Gene Name
glypican 3
Official Symbol
GPC3
Gene ID
2719
Synonyms
GPC3; glypican 3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2; glypican-3; secreted glypican-3; glypican proteoglycan 3; intestinal protein OCI-5; heparan sulphate proteoglycan; CD28; Chimeric antigen receptor; T cell; Retrovirus; CARs; Lentivirus; chimeric T cell receptors; chimeric immunoreceptors; chimeric antigen receptors; scFv; 9C2

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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CONTACT US

USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-381-2994
Fax: 1-631-207-8356
Email:

Europe
Ringstrasse 4, 64401 Gross-Bieberau, Germany
Tel: 44-207-097-1828

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