Flow Cytometry for Cell Surface Glycosylation Analysis

Equipped with state-of-the-art technologies as well as substantial experience and expertise in the field of glycosylation analysis, Creative Biolabs employs lectin-based flow cytometry technology for glycosylation profiling of interested cell lines. We offer both custom and standard analysis according to your needs and deliver the project report in a timely manner.

Importance of Glycosylation

Glycosylation of proteins is one of the most important post-translational modifications (PTMs), with more than 50% of all human proteins estimated to be glycosylated. The site-specific nature of this modification is involved in numerous essential biological functions including cell proliferation, differentiation and migration, cell-cell recognition and interaction, and signal processes that regulate human development, immunity and disease. The glycans found in humans are predominantly attached to the protein in two ways: 1) N-glycan, a GlcNAc residue is added to Asn within Asn-X-Ser/Thr, where X can be any amino acid except proline; 2) O-glycan, a GalNAc residue is attached to the hydroxyl group of Ser or Thr. Glycoproteins on the cell surface typically undergo glycosylation in the endoplasmic reticulum-Golgi pathway where a sequence of regulated events occurs, resulting in creating highly complex but specific glycan structures on the proteins.

Fig.1 Flow cytometry analysis of surface glycosylation of Mel202 cells. (Surman, 2019)

Glycosylation Profiling of Cell Lines at Creative Biolabs

In terms of the extensive experience in glycosylation research, we offer top-quality glycosylation analysis of interested cell lines. The routine includes sample processing, protein labeling, flow cytometry assay and data analysis. Our continually expanding range of facilities and equipment allows us to offer a wide range of multiple services, including but not limited to:

Cell culture service

We offer cell culture service based on every requirement of our clients. Besides, we also have diverse cell lines, including tumor cells, immune cells and other cell lines, and thus clients can choose according to their own demands. Moreover, we provide service to treat cell lines with different drugs based on the specifications of our clients.

Glycosylation profiling by lectin-based flow cytometry

With Ph.D. level scientists and extensive experience in glycosylation analysis, we are proficient in glycosylation profiling of interested cell lines by lectin-based flow cytometry.

Highlights

Hundreds of lectins for choice, such as LCA, PNA, SNA and LEL, etc.
Diverse cells can be analyzed, including tumor cells, immune cells and other cell lines
High flexibility and cost-effectiveness
Reliability and information security

Powered by our advanced high-resolution analytical platforms and seasoned technical personnel, we are fully competent and dedicated to serving as your one-stop-shop for glycosylation profiling. With proven experience in glycoscience, Creative Biolabs is an ideal company to entrust with your business. We offer turn-key or a la carte services customized to our client’s needs. Please contact us for more information and a detailed quote.

Reference

Surman, M.; et al. An insight into the proteome of uveal melanoma-derived ectosomes reveals the presence of potentially useful biomarkers. International journal of molecular sciences. 2019, 20(15):3789

For Research Use Only.

Flow Cytometry for Cell Surface Glycosylation Analysis

Importance of Glycosylation

Glycosylation Profiling of Cell Lines at Creative Biolabs

Highlights

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