Custom Oligosaccharide Synthesis

Advances in carbohydrate chemistry have certainly made common oligosaccharides much more accessible. Equipped with world-leading technology platforms and professional scientific staff in glycan synthesis, Creative Biolabs is committed to offering high-quality custom oligosaccharide and oligosaccharide derivatives synthesis services to promote the progress of oligosaccharide related projects.

Importance of Oligosaccharide

Oligosaccharide sequences are found in numerous natural compounds and constitute the core of many therapeutics. They are involved in a wide range of biological processes, including bacterial and viral infection, cancer metastasis, the blood-clotting cascade, and many other crucial intercellular recognition events. The presence of glycans in glycoproteins, glycolipids, glycosaminoglycans, and other conjugates presents a treasure of potential information on cellular differentiation and condition. Over half of all proteins in the human body are N- or O-glycosylated, and cell surfaces present a rich multitude of glycolipids and glycosaminoglycans. Oligosaccharides carrying information on biological significance are found in body fluid, on cell surfaces, and within cells.

Applications of Oligosaccharides in Glycomedicine

A major obstacle in glycomedicine is the lack of pure and structurally well-defined oligosaccharides. These compounds are usually in low concentrations and in microheterogeneous forms, which make the isolation and characterization complicated. Based on chemical or enzymatic synthesis methods, well-defined oligosaccharides have been obtained in many cases. Such compounds are increasingly used to address important problems in glycobiology research and for drug discovery. For example, chemoenzymatic approach for the preparation of a library of over 400 oligosaccharides using array technology has been employed for profiling the specificity of a diverse range of glycan-binding proteins.

Synthetic oligosaccharide epitopes offer promising possibilities for the development of vaccines for the prevention of infectious diseases, including human immunodeficiency virus (HIV), Haemophilus influenzae type b, Plasmodium falciparum, Cryptococcus neoformans, Vibrio cholerae, Streptococcus pneumoniae, Neisseria meningitides, Candida albicans, and Bacillus anthracis. These oligosaccharides have also been employed for the development of therapeutic vaccines for cancer. The over-expression of oligosaccharides, such as Tn antigen, is a common feature of oncogenic transformed cells. A broad of preclinical and clinical studies have demonstrated that antibodies against carbohydrate-associated tumor antigens are able to eliminate circulating tumor cells and micro-metastases in cancer patients.

Custom Oligosaccharide Synthesis at Creative Biolabs

Despite ongoing challenges, tremendous progress has been made in recent years for the synthesis of oligosaccharide. With the latest instruments and professional staff, Creative Biolabs utilizes our advanced synthesis technology platforms and specialized expertise to serve our global clients with high-quality oligosaccharide and oligosaccharide derivative, including but not limited to:

• Heparan Sulfate/Heparin Oligosaccharides
• Chondroitin Sulfate
• N-Linked Oligosaccharides
• Milk Oligosaccharides

Highlights

• Synthesis of natural and non-natural oligosaccharides
• Large quantity, up to 10 g scale
• Ranging from 4-mer to 18-mer
• Various chemical modifications at the part of sugar chain, including bromoacetamidyl modification
• Introduction of fluorescent labels or affinity tag, like biotin at the reducing end or nonreducing end of the oligosaccharides
• Stable isotopically labeled oligosaccharides with ¹³C-, ²H- or both ¹³C- and ²H

Oligosaccharides are increasingly used as probes for biological research and as lead compounds for drug and vaccine discovery. Creative Biolabs is pleased to share our technology and extensive experience in oligosaccharide synthesis to meet customers’ needs from early research to commercial production. Please contact us for more information and a detailed quote.

Reference

1. Boltje, T.J.; et al. Opportunities and challenges in synthetic oligosaccharide and glycoconjugate research. Nat Chem. 2009, 1(8):611-22.

Related Services:

1. Custom Monosaccharide Synthesis
2. Custom Nucleotide Sugar Synthesis
3. Custom Glycolipid Synthesis
4. Custom Polysaccharide Synthesis
5. Custom Carbohydrate Antigen Synthesis