Mass spectrometry (MS) is a powerful analytical technique that is widely used in the field of glycomics and glycobiology. Different separation methods such as capillary electrophoresis (CE) combined with MS is used to provide highly informative fragments for structural identification of glycoproteins. Based on MS technique, Creative Biolabs is able to offer the first-rate service of glycoprotein analysis for our customers.

Glycoproteins Analysis Based on MS

MS is an analytical technique that ionizes chemical species and sorts the ions based on their mass-to-charge ratio. It is widely used to detect and identify unknown compounds within a sample, quantify known materials, as well as to elucidate the structure and chemical properties of different molecules. An effective separation technique is required to increase sensitivity by reducing the number of species that endure simultaneous ionization, thus improving the high-quality characterization of the high complexity of glycan sample. Especially in glycoprotein analysis, MS in conjugation with different separation methods, such as CE, hydrophilic interaction chromatography (HILIC), and ion mobility, has emerged as a popular tool for glycoprotein analysis, providing highly informative fragments for structural identification of glycoproteins. For example, CE is a useful tool for glycan separation due to its high sensitivity and speed, as well as its isomer resolution and superior peak capacity. CE-MS is a useful tool in determining site-specific glycan micro-heterogeneity of glycoproteins at the glycopeptide level, as well as in peptide mapping and glycoform. HILIC provides better separation of hydrophilic glycopeptides as well as regular peptides but not glycopeptide isomers. ESI-MS and HILIC-MS are both used to determine the characterization of protein glycosylation site(s) and occupancy. Besides, ESI is also appropriate for the direct analysis of acidic glycans for MS and tandem MS. MALDI-MS is used extensively for analysis of glycan classes.

A schematic overview of experimental approaches for the systematic characterization of glycoproteins by mass spectrometry-based proteomic analysis. Fig. 1 A schematic overview of experimental approaches for the systematic characterization of glycoproteins by mass spectrometry-based proteomic analysis (Ruiz-May, 2012).

MS Analysis Services in Creative Biolabs

Professional experts in Creative Biolabs have worked on glycoproteins analysis research for many years. Hence, we are very confident to offer high-quality and comprehensive services of glycoproteins analysis for global clients. Our MS technology is usually combined with various separation techniques such as CE, ESI, HILIC, MALDI, making it a sensitive and rapid glycoproteins analysis platform. Based on these techniques, we offer a full range of glycoproteins analysis services including Glycan Profiling, Glycomic Profiling, Glycosylation Site Mapping, and Glycan Sequencing.

Features of Our Services

  • Experienced expert team
  • Advanced technique platforms
  • Providing accurate and comprehensive analysis data as quickly as possible
  • High quality and low-cost services

Equipped with diverse technologies and the best scientists, Creative Biolabs is capable of providing a full series of glycoprotein analysis services for clients all over the world. Based on MS technology, we are able to provide high-quality and low-cost services including glycan profiling, glycomic profiling and glycosylation site mapping. If you are interested in our MS analysis services, please feel free to contact us for more details.

Reference

  1. Ruiz-May, E.; et al. Analytical technologies for identification and characterization of the plant N-glycoproteome. Front Plant Sci. 2012, 3(150):1-8.

Related Services:

  1. Lectin Microarray
  2. LC-ESI-MS
  3. HPAEC-PAD
  4. RP-HPLC
  5. MALDI-TOF MS
  6. HPLC
  7. UHPLC/FLD/Q-TOF

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