Glycan analysis is very challenging because of the extremely high number of possible isomers that can be formed by naturally occurring monosaccharides. Over the last years, the analysis of glycoproteins and complex glycans has made great progress mainly because of the improvement of analytical instruments, such as nuclear magnetic resonance (NMR) spectroscopy. With years of experience in the condition optimization of NMR spectroscopy for glycan analysis, Creative Biolabs has gained significant knowledge to offer high-quality service. We are happy to share our knowledge and passion in this field to facilitate our customers’ research and project development.
Glycans, most often in the form of glycoconjugates such as N- or O-linked glycoproteins, proteoglycans, and polysaccharides, are an important class of biomolecules that are involved in molecular recognition, protein trafficking, regulation, and inflammation among other things. The rapid evolution in glycomics area has indicated that the structure of glycans from glycoproteins is highly relevant for their function. To comprehensively understand the function of glycoproteins, the glycan structures must be determined as accurately as possible.
Many methods for glycan analysis have been developed depending on the analytical methods used, data on the composition, sequence, linkage, branching, and anomericity. For instance, mass spectrometry (MS)-based methods can be used to carry out for glycan profiling rapidly, but it relies on knowledge of conserved structures and to some extent on known biosynthetic pathways. NMR spectroscopy of glycans leads to breakthrough in the glycan analysis. NMR spectroscopy can determine stereochemical relationships of completely novel sugars and in a reliable way also the anomeric configuration. However, limited sensitivity in high molecular mass systems is the main disadvantage in the application of NMR spectroscopy. The recent improvements in sensitivity include cryo-probe technology, “single-scan 2D techniques”, and dynamic nuclear polarization, promising efficient application of NMR spectroscopy for carbohydrate analysis. Solution NMR spectroscopy is an ideal technique to characterize the structure and dynamics of conformationally labile systems. Polysaccharides themselves are conformationally labile due to a lack of short- or long-range stabilizing interactions, but all members of a conformationally labile moiety often contribute to observable NMR parameters. Given the intrinsic dynamic nature of glycans, NMR spectroscopy can afford exquisite structural information at the atomic detail, not accessible by other techniques.
Fig.1 NMR data of native glycans. (Fellenberg, 2013)
To unravel the biological roles of glycans, many approaches have been developed. Among them, NMR spectroscopy is a very powerful tool for the analysis of glycoprotein and complex glycans. Our professional technical scientists are proficient in applying NMR spectroscopy to determine the primary and tertiary structure of glycans from glycoprotein and polysaccharides. The sensitivity has been highly improved by the optimization of buffer suppression and sample preparation. Moreover, this technique is nondestructive, leaving the sample intact for further analysis.
Glycobiology turned to be a fertile field for NMR spectroscopy. Powered by our world-leading technology platforms and experienced technical personnel, Creative Biolabs is fully competent and dedicated to serving as your one-stop-shop for glycan analysis by NMR spectroscopy. If you are interested in NMR spectroscopy for glycan analysis, please don’t hesitate to contact us for more detailed information.