Custom Glycan Synthesis

A Source of Your Glycans and Glycan Derivatives

Glycans are usually attached to lipids and proteins to form glycolipid and glycoproteins. It is involved in a variety of biological process including immunological response, cell-cell communication, and inflammation reaction. Hence it is very important to synthesize glycans with high-purity to investigate glycobiology. With extensive experience in glycan synthesis, Creative Biolabs offers a full range of custom glycan synthesis services with the highest quality and the most competitive price.

Background of Glycan Synthesis

Glycans are defined as the compounds consisting of a large number of monosaccharides linked glycosidically. Usually, they are found to attach to proteins and sphingolipids in biological systems and are involved in important functions such as inflammation, the immunological response, metastasis, and bacterial and viral infection. Due to the inherent chemical properties of oligosaccharides, glycan synthesis is more complex than the other major classes of biomolecules such as proteins and nucleic acids. In biological synthesis systems, glycans are heterogeneous and their synthesis is no-template while proteins and nucleic acids are produced in homogeneous forms based on the template strand. Chemical synthesis of specific complex oligosaccharide structures that are not easily isolated from natural material or accessible via enzymatic routes usually involves a considerable number of synthetic steps.

Fig.1 Various transformations in typical complex glycan synthesis. (Seeberger, 2009)

Custom Glycan Synthesis Service at Creative Biolabs

Different strategies have been developed to make custom glycan synthesis possible, such as chemical synthesis method and chemoenzymatic synthesis. In order to increase the production of glycan, chemical synthesis method has been used to produce homogeneous glycans in larger quantities than are available from most cellular production systems. Besides, enzymes can be combined with chemical methods to further simplify the synthesis process of glycan. Moreover, chemical synthesis can be further used to incorporate polysaccharides into homogeneous glycoproteins.

Based on various synthesis strategies, Creative Biolabs offers our clients comprehensive services for custom glycan synthesis, including but not limited to:

• Custom Monosaccharide Synthesis
• Custom Oligosaccharide Synthesis
• Custom Nucleotide Sugar Synthesis
• Custom Glycolipid Synthesis
• Custom Polysaccharide Synthesis
• Custom Carbohydrate Antigen Synthesis

Highlights

• Glycans with chemical modifications
• Glycans with stable isotope labeling, such as ²H, ¹³C, ¹⁵N, or ¹⁸O
• Strict quality control by various instruments, such as nuclear magnetic resonance (NMR), liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS), etc.
• A powerful platform that allows to provide a comprehensive custom glycan synthesis service
• A variety of glycan synthesis strategies including enzymatic and chemical methods
• A professional expert staff comprised of prominent academic and industry leader

Creative Biolabs is committed to offering our customers a comprehensive service of custom glycan synthesis at an affordable price within tight deadlines. Over the past years, we have helped many customers attain their on-demand synthesis of custom glycans, including N-glycans, O-glycans, glycosaminoglycans, GPI-anchors, and glycoconjugates. Please feel free to contact us for more details.

Reference

1. Seeberger, P.H.; et al. Chemical and enzymatic synthesis of glycans and glycoconjugates. Essentials of Glycobiology. 2009.

Related Services:

1. Custom Glycopeptide Synthesis
2. Custom Glycoprotein Synthesis
3. Oligosaccharide Library Development