Creative Biolabs is a forward-looking company that has established a comprehensive and advanced modification platform. We provide the latest technologies and the most comprehensive services in phosphorylation modification, including carbohydrate phosphorylation.
Phosphorylation is one of the most extensively and diligently studied post-translational modification (PTM) which orchestrates a variety of cellular functions like cell growth, differentiation, and apoptosis. Protein kinases and phosphates are the enzymes that phosphorylate and dephosphorylate the proteins, respectively. These enzymes work in a balanced state to regulate the function of proteins. Phosphorylation imparts conformational changes in the protein thereby modify its affinity for another protein, activates or deactivates an enzyme protein, or facilitates its degradation by the ubiquitin-proteasome complex.
The hydroxyl groups on the polysaccharides are replaced by a phosphate group, known as phosphorylation of polysaccharides. Phosphoric acid and its anhydride, phosphorus pentoxide (P2O5), phosphorus oxychloride (POCl3), phosphate are commonly used for the phosphorylation of polysaccharides. The introduction of the phosphate group will increase the immunosuppressive activity of polysaccharides. This advantage makes polysaccharide phosphorylation have great significance. It showed that dextran phosphorylation could significantly promote mouse spleen lymphocyte mitosis, enhance the expression of B lymphocytes and dendritic cells on the surface of CD86 and CD69, but also could promote IL-10 secretion. The immunomodulatory activity of phosphorylated polysaccharides was related to the absorption of calcium ions.
Fig.1 Fourier-transform infrared (FTIR) spectroscopy of Radix Hedysari (RHP) and its phosphorylated derivatives. A RHP; B phosphorylated polysaccharides (RHPP)-3. (Wei, 2012)
Creative Biolabs has long been devoted to carbohydrate modification. Years of experience make us confident in providing high-quality service of carbohydrate phosphorylation to meet our global customers' detailed demands. Our Ph.D. level scientists will work closely with you and tailor the appropriate solution for your research. Our services include phosphorylation modification and modification analysis, such as sample separation by high-performance liquid chromatography (HPLC) and mass spectrometry (MS) data analysis. Creative Biolabs will forever provide the best services for our global customers according to their detailed demand with pleasure.
Creative Biolabs offers the most comprehensive carbohydrate phosphorylation modification service to meet customers' complex and puzzling problems in the field of carbohydrates. Our advanced platform provides various services, from which you can always find the best match for your particular research. Please feel free to contact us for more information.