Glycoprotein quantification is one principle test in the researches of glycosylated proteins, which has become hot spots in the pharmaceutical industry. As a leader in the pharmaceuticals field, Creative Biolabs combines first-in-class technologies with experienced scientists to explore well-established platforms for glycoprotein production, optimization, quantification, and analysis. At present, we have won a good reputation among our worldwide customers for successfully accomplishing numerous challenging projects in glycoprotein quantification based on the values of quality, timing, and price.
Glycosylation is an integral part of proteomics, which is a very critical modification of therapeutic proteins, known to significantly modulate yield, bioactivity, solubility, stability against proteolysis, immunogenicity, and clearance rate from circulation. The glycan groups of glycoprotein mediate interactions of all cells, including reproductive cells, and regulate fundamental biological, chemical, and physical processes including evolution. A large number of key molecules and therapeutic proteins, such as vaccines, antibodies, and enzymes, need glycans to have full biological activity. There is an increasing need for high throughput and quantitative analysis methods to characterize blood, serum or plasma glycoproteins for therapeutic or diagnostic purposes. However, the enormous complexity of glycoprotein makes their comprehensive analysis be an as-yet-unresolved technical challenge. Glycoprotein quantification is also a big puzzle because of the substoichiometric relation between glycosylation and the protein.
Creative Biolabs employs several efficient technologies for glycoprotein quantification. Our efficient strategies including but not limited to:
Lectins are widely used in glycosylation engineering because of the ability to bind mono- or oligosaccharides specifically. Through lectin purification, followed by a labeling procedure, we can test the quantification of a specific glycoprotein relatively, which is the simplest method. Moreover, lectin chromatography can be used for comparative quantification of a specific glycoprotein in several samples.
LC-MS has become a standard method with broad projected clinical applicability in the quantification of glycoproteins, which provides unparalleled sensitivity and selectivity for detection and quantification. Quantification of glycopeptides was performed using multiple reaction monitoring (MRM).
NP-HPLC has previously been used for relative quantification of 2-AB-labeled oligosaccharides in some samples.
In addition, we can provide a variety of other quantitative technologies, including but not limited to the ones listed above.
Fig.1 Quantitative proteomic schematic of serum glycoproteins (Gao, 2015).
Quantitative protein profiling has been recognized as an important approach for profiling the physiological state or pathological state of cells or organisms. Creative Biolabs has successfully completed a lot of therapeutic glycoprotein projects, including numerous glycoprotein quantification cases. As your best partners, we are glad to revolutionize your particular project and promote your success. If you have special project requirements, please contact us for further consultation.