Reversed-Phase-High Performance Liquid Chromatography (RP-HPLC)

Creative Biolabs is a world-leading services provider in the field of glycoprotein analysis. Scientists in Creative Biolabs have engaged in the research of glycoprotein analysis for many years. We are confident in offering global customers a full range of glycoprotein analysis services based on our advanced RP-HPLC technique.

Glycoproteins Analysis Based on RP-HPLC

RP-HPLC has a non-polar stationary phase and an aqueous, moderately polar mobile phase. This method is very efficient to separate the polar compounds due to the interaction between polar compounds with polar mobile phase. RP-HPLC experimental system is usually composed of an n-alkylsilica-based sorbent. Solutes are eluted from sorbent with gradients of increasing concentrations of an organic solvent such as acetonitrile containing an anionic modifier. Complex mixtures of peptides and proteins can be routinely separated. Separations can be easily performed by changing the gradient slope of solvents, the operating temperature, the ionic modifier, or the organic solvent composition. The separated fractions can be used for further characterization.

RP-HPLC is a commonly used method for the analysis and purification of peptides, proteins, and glycoproteins. Monosaccharide composition and content can be determined by using the RP-HPLC separation of p-aminobenzoic ethyl ester derivatives of neutral and amino sugars released from glycoproteins. Moreover, a study has shown that RP-HPLC method has the best coverage for glycopeptide analysis based on the number of detected glycosylation sites and has identified glycopeptides in three model glycoproteins, superior to chromatographic and/or enrichment approaches such as HILIC, and lectin affinity.

Fig. 1 Monosaccharide analysis of fractions 23-27 from RP-HPLC separation of a tryptic digest of recombinant tissue plasminogen activator (Miyazaki, 2000).

RP-HPLC Analysis Services

RP-HPLC is an effective tool that is widely used for Glycan Profiling. Based on this method, carbohydrates, glycopeptides, and glycoproteins can be effectively and rapidly separated and subjected to further characterization. Our professional team has worked on glycoprotein analysis for many years. We are confident to offer a full series of glycoprotein analysis services with the best quality and the most competitive prices. So far, we have served hundreds of customers and won the customer's unanimous praise and recognition within a few years.

Features of Our Services

• A strongly practical and fully experienced technical team
• The latest technique platforms
• Providing accurate and comprehensive analysis data as soon as possible
• High-quality and affordable service
• Whole-process technical trace service

Creative Biolabs has accumulated extensive experience in glycoprotein analysis and is able to provide high-quality glycoprotein analysis services. Based on RP-HPLC, we can offer the best glycan profiling services for global clients. If you are interested in our RP-HPLC analysis services, please feel free to contact us for more details.

Reference

1. Miyazaki, K. Glycoproteins: liquid chromatography. Encyclopedia of Separation Science. 2000, (6):2960-2965.

Related Services:

1. Lectin Microarray
2. MS
3. LC-ESI-MS
4. MALDI-TOF MS
5. HPLC
6. HPAEC-PAD
7. UHPLC/FLD/Q-TOF
8. FTIR
9. NMR
10. SPRi
11. TLC
12. Flow Cytometry
13. GC-MS
14. Imaging Mass Spectrometry
15. Glycobiology Microarray