High-throughput Glycan Screening Service

As a leading specialist in glycoscience, Creative Biolabs is proud to launch our high-throughput glycan screening service to help our worldwide clients with their cutting-edge research projects. By applying our staffs’ experience and a proven quality system, our scientists employ two strategies, including UHPLC-compatible fluorescence detector (UHPLC-FLD) and fluorescence-labeled lectin-based glycan assay for high-throughput glycan screening. Moreover, we also offer high-quality high-throughput titer assays for antibody/Fc fusion protein and cell line development.

High-throughput Glycan Screening Service at Creative Biolabs

Glycoproteins play key roles in various molecular and cellular functions and are among the most difficult to analyze biomolecules on account of their microheterogeneity, low abundances, and non-template-driven synthesis. The stability, serum half-life, immunogenicity, and biological activity of therapeutic glycoproteins are regulated by their glycosylation profile. Thus, there is increasing demand for the qualitative and quantitative characterization of glycosylation on glycoproteins in all biologics development stages. Creative Biolabs has developed two simple and efficient strategies for high-throughput glycan screening on antibodies, Fc fusion proteins, non-Fc fusion proteins, or in glycoengineered cell lines. Minimal samples can be used for high-throughput glycan screening in all biologics development stages. Our strategies can automate the high-throughput glycan screening on large sample sets of crude cell culture supernatants and purified intact glycoproteins.

High-throughput glycan screening by using UHPLC-FLD

Samples are analyzed by UHPLC-FLD. As short as 1 week for up to 100 samples.
Samples as crude as clarified cell culture media can be processed. Our seasoned scientists offer comprehensive analysis of various time points and conditions to support our clients’ cell culture development program's needs.

High-throughput Glycan Screening Service 1

High-throughput glycan screening by glycan assay

Fluorescence-labeled lectin-based glycan assay.
Detection of different types of important glycans, including high mannose, fucose, galactose, and sialic acid.
Screening on antibodies, Fc fusion proteins, non-Fc fusion proteins, or in glycoengineered cell lines.
The whole process takes roughly 2 hours.

Features of Our High-throughput Glycan Screening Service Including but not Limited to:

Fluorescence labeled lectins to detect different types of glycosylation
High mannose, fucose, galactose, and sialic acid identification
Discrimination of 2,3 and 2,6 N-sialylation patterns
Both crude cell culture supernatants and intact glycoproteins are available

High-throughput Glycan Screening Service 2

Applications of High-throughput Glycan Screening Including but not Limited to:

Media optimization
Glycan monitoring during bioreactor runs
Accelerating target clone screening
Glycoengineered cell line screening

Titer Assays

Besides high-throughput glycan screening, scientists at Creative Biolabs have developed several efficient titer assays, including antibody/Fc fusion protein titer assay and high-throughput titer assay for cell line development.

Antibody/Fc fusion protein titer assays
Our antibody/Fc fusion protein titer assay enables the rapid titer analysis of antibodies/Fc fusion proteins in small volumes of cell culture supernatant. This assay can be analyzed automatically in 25 minutes in a 384-well plate without sample preparation.
High-throughput titer assay for cell line development
Our automated high-throughput titer assay for cell line development has several advantages, including high speed, high accuracy, and low price.

With proven experience in high-throughput glycan screening and titer assay, Creative Biolabs is an ideal company to entrust with your business. Through our high-throughput system, our clients could accelerate their research with reduced project costs and timelines. For more detailed information, please feel free to contact us or directly send us an inquiry.

Reference

Yang, X.; et al. Ultrafast and high-throughput N-glycan analysis for monoclonal antibodies. InMAbs, 2016, Vol. 8, No. 4, pp. 706-717. Taylor & Francis.

For Research Use Only.

High-throughput Glycan Screening Service