Glycoprotein synthesis is a complicated process because this process is no-template synthesis and glycan inherent structure is complex. Creative Biolabs has rich experience in glycoprotein synthesis (up to 150 amino acids in length) using chemical methods and we are confident to provide a full range of high-quality custom glycoprotein synthesis services with affordable prices.
Glycoprotein is formed by a covalent linkage between glycans and proteins. Glycoprotein synthesis is a very complicated process due to the inherent complexity of the glycan structure. Oligosaccharides attached to proteins consist of highly branched biopolymers. They attach to the peptide backbone mainly through the amide nitrogen of asparagine (N-linked) or the oxygen on serine or threonine (O-linked). Besides, there is no biological template for oligosaccharides, unlike peptides and nucleic acids. During the process of homogeneous glycoproteins synthesis, there are three major problems that should be considered: 1) homogeneous glycans obtaining; 2) production of the protein backbone; 3) linkage between glycan and protein backbone. In order to address these problems, a number of methods have been developed over the past years, including both chemical and enzymatic approaches such as native chemical ligation, bacterial oligosaccharyltransferase, glycoprotein remodeling, and in vivo suppressor tRNA technology.
Fig.1 Methods developed for the synthesis of homogeneous glycoproteins: (1) glycopeptide ligation; (2) glycoprotein remodeling; and (3) in vivo suppressor tRNA technology. (Liu, 2005)
Creative Biolabs is a world-leading service provider of custom synthesis including custom glycoprotein synthesis. Several advanced strategies, including glycoprotein remodeling, native chemical ligation (NCL), expressed protein ligation (EPL), Staudinger ligation and sugar-assisted ligation, are employed. Our specialists have developed a highly specialized platform as well as various comprehensive and innovative protocols for glycoprotein synthesis, through rational design from glycan position to glycan structure to glycoprotein optimization. Our professional technical staff has extensive experience in glycoprotein synthesis (up to 150 amino acids in length).
We proudly offer the most comprehensive custom glycoprotein synthesis services to global customers and our professional services have assisted a lot of customers to overcome multiple challenges in glycoprotein synthesis. For more detailed information, please feel free to contact us or directly send us an inquiry.