Custom Glycoprotein Synthesis Services
Glycoprotein synthesis is a complicated process because this process is no-template synthesis and glycan inherent structure is complex. Creative Biolabs has rich experience in glycoprotein synthesis (up to 150 amino acids in length) using chemical methods and we are confident to provide a full range of high-quality custom glycoprotein synthesis services with affordable prices.
Background of Glycoprotein Synthesis
Glycoprotein is formed by a covalent linkage between glycans and proteins. Glycoprotein synthesis is a very complicated process due to the inherent complexity of the glycan structure. Oligosaccharides attached to proteins consist of highly branched biopolymers. They attach to the peptide backbone mainly through the amide nitrogen of asparagine (N-linked) or the oxygen on serine or threonine (O-linked). Besides, there is no biological template for oligosaccharides, unlike peptides and nucleic acids. During the process of homogeneous glycoproteins synthesis, there are three major problems that should be considered: 1) homogeneous glycans obtaining; 2) production of the protein backbone; 3) linkage between glycan and protein backbone. In order to address these problems, a number of methods have been developed over the past years, including both chemical and enzymatic approaches such as native chemical ligation, bacterial oligosaccharyltransferase, glycoprotein remodeling, and in vivo suppressor tRNA technology.
Fig.1 Strategies for chemical synthesis of glycoproteins.1
Custom Glycoprotein Synthesis Services
Creative Biolabs is a world-leading service provider of custom synthesis including custom glycoprotein synthesis. Several advanced strategies, including glycoprotein remodeling service, native chemical ligation (NCL), expressed protein ligation (EPL), Staudinger ligation and sugar-assisted ligation, are employed. Our specialists have developed a highly specialized platform as well as various comprehensive and innovative protocols for glycoprotein synthesis, through rational design from glycan position to glycan structure to glycoprotein optimization. Our professional technical staff has extensive experience in glycoprotein synthesis (up to 150 amino acids in length).
Features of Our Services Including but Not Limited to:
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High-quality service——we make a promise to provide a comprehensive custom synthesis service from the project receiving to the end.
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Professional technical team——our experts have extensively specialized in the field of glycoprotein synthesis.
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Rapid and effective solutions——professional technical team keeps track of project progress and give guidance in real-time.
We proudly offer the most comprehensive custom glycoprotein synthesis services to global customers and our professional services have assisted a lot of customers to overcome multiple challenges in glycoprotein synthesis. For more detailed information, please feel free to contact us or directly send us an inquiry.
Published data
Glycoproteins are central to many vaccines. Synthesis of glycoproteins by customization, for example, helps to improve the safety and efficacy of carbohydrate vaccine candidates. Manipulating and doping glycosidic residues on glycoproteins and modifying them in a site-specific manner are challenges in glycoprotein synthesis. This study introduces us to several methods including chemical total synthesis and chemoenzymatic synthesis method. The chemoenzymatic synthesis method has several advantages and is used to control the sequences through different strategies.
Some strategies have been developed to synthesize glycoproteins with well-defined structures including targeting and modification of amino acid residues, chemical ligation strategies, and chemoenzymatic transformation (i.e., glycoprotein remodeling) using enzymes. Among these, glycoprotein remodeling is an attractive approach to synthesizing homogeneous glycoproteins. The mild and protecting group-free conditions used for enzymatic reactions and the stereocontrol and precise regions achieved using enzymes make glycoprotein synthesis more convenient.
These strategies described in the paper enable the synthesis of glycoproteins with different glycosylation patterns and have also been used for the synthesis of vaccines with different carbohydrate conformations, densities, and shapes.
Fig.2 Strategies for the production of homogeneous glycoproteins.1
FAQs
Q1: What is the significance of glycoprotein synthesis?
A1: Glycoprotein plays a very critical role in various physiological activities. The microscopic heterogeneity of glycan structures in natural glycoproteins hinders the study of conformational relationships between glycan structures and glycoprotein functions. With the continuous advances in protein synthesis and glycan engineering technologies, several protein linkage strategies and glycosidic bond construction strategies are developed to precisely synthesize homogeneous glycoproteins. These synthesized glycoproteins have potential significance in glycoprotein function studies, vaccine development, exploration of disease molecular mechanisms, disease diagnosis, and therapeutic studies.
Q2: What are the advantages of chemical and enzymatic synthesis methods in synthesizing homogeneous glycoproteins?
A2: Chemical and enzymatic synthesis methods are effective in synthesizing glycoproteins with a homogeneous glycan type. They provide a basis for studying the biological properties and structure of glycoproteins, and also by introducing carbohydrate fractions or non-natural amino acids for additional glycoprotein studies.
Q3: How long does it typically take to custom synthesize glycoproteins?
A3: The time required for this process varies depending on the complexity of the glycoprotein, the synthesis method, and other factors. Our experienced team will develop a detailed synthesis strategy after clarifying the client's needs. The entire project cycle can range from a few weeks to a few months.
Customer Review
Mature Glycoprotein Synthesis Solution
"We appreciated Creative Biolabs for their mature solution to synthesize glycoproteins. They helped us to synthesize glycoproteins with a homogeneous sugar pattern, which accelerated our research process. Thank them for their excellent service."
Extensive Practical Experience in Glycoprotein Synthesis
"We could see that Creative Biolabs has a wealth of knowledge and experience in glycoprotein synthesis. The advanced technology coupled with the efficient synthesis solution made the whole collaboration very efficient and comfortable. All in all, this collaboration was a very good experience."
Reference
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Adamo, Roberto, et al. "Synthetically defined glycoprotein vaccines: current status and future directions." Chemical science 4.8 (2013): 2995-3008. Distributed under Open Access license CC BY 3.0, without modification.
For Research Use Only.
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