MetaVital™ Rat Glutathione S-Transferase Kappa 1 (GSTK1) ELISA Kit

CAT#: ITS-0326-WB38
Product Type: ELISA Kit
Species: Rat
Target: Rat Glutathione S-Transferase Kappa 1 (GSTK1)
Brand: MetaVital™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures rat GSTK1 protein levels in biological samples using a sensitive sandwich ELISA method.
Brand
MetaVital™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
MetaVital™ Rat Glutathione S-Transferase Kappa 1 (GSTK1) ELISA Kit is a highly sensitive immunoassay designed for the quantitative detection of GSTK1 protein in rat biological samples. Based on a sandwich ELISA principle, this kit enables reliable measurement of GSTK1 concentrations in serum, plasma, tissue lysates, and cell culture samples, supporting studies of oxidative stress regulation, metabolic detoxification, and mitochondrial function.
Features
High-affinity antibodies ensure precise and specific recognition of rat GSTK1, minimizing cross-reactivity and enabling reliable protein quantification in complex biological matrices.
Sandwich ELISA format delivers excellent sensitivity and reproducibility across a broad dynamic detection range for consistent protein analysis.
Optimized reagents and standardized protocol allow efficient processing of multiple samples with minimal hands-on time and experimental variability.
Compatible with various rat-derived sample types including serum, plasma, cell lysates, and tissue homogenates for versatile research applications.
Process Relevance
Quantifying GSTK1 levels enables assessment of cellular detoxification capacity and oxidative stress responses in experimental models.
Application Stage
Drug discovery, toxicology studies, metabolic research, and biomarker evaluation in preclinical rat models.
Applications
Quantitative detection of GSTK1 in rat serum, plasma, tissue homogenates, and cell lysates for oxidative stress studies, metabolic enzyme profiling, toxicology research, ADME profiling studies, and biomarker analysis
Target
Rat Glutathione S-Transferase Kappa 1 (GSTK1)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Oxidative stress biology, pharmacokinetics (ADME), detoxification mechanisms, mitochondrial metabolism, pharmacology, toxicology, metabolic disease research, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using endpoint absorbance detection in a sandwich ELISA format.
Assay Range
0.156 ng/ml - 10 ng/ml
LOD
< 0.07 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Glutathione S-transferase kappa 1 (GSTK1) is a mitochondrial enzyme involved in cellular detoxification and protection against oxidative damage. It catalyzes the conjugation of reduced glutathione to reactive electrophilic compounds, facilitating their elimination. GSTK1 is particularly relevant in metabolic tissues where oxidative stress and xenobiotic metabolism are actively regulated.
Pathways
GSTK1 participates in the glutathione metabolism and detoxification pathways, supporting cellular defense against reactive oxygen species and toxic electrophiles. It functions downstream of oxidative stress signaling and cooperates with glutathione synthesis enzymes and antioxidant systems to maintain mitochondrial redox balance.
For Research Use Only | Not For Clinical Use
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