CytoFinal™ Human Aspartate Aminotransferase, Cytoplasmic / AST (GOT1) ELISA Kit

CAT#: ITS-0326-WB104
Product Type: ELISA Kit
Species: Human
Target: Cytoplasmic Aspartate Aminotransferase (AST1)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures cytoplasmic AST (GOT1) levels in human biological samples with high precision and specificity.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Human AST (GOT1) ELISA Kit provides a reliable in vitro method for detecting and quantifying cytoplasmic Aspartate Aminotransferase in human serum, plasma, tissue lysates, and other biological fluids. Using a sensitive sandwich ELISA format, this kit ensures accurate measurement of AST1 concentrations with minimal cross-reactivity, supporting diverse biomedical research applications.
Features
High sensitivity detection allows precise quantification of low-abundance cytoplasmic AST in human samples with excellent reproducibility.
Strong specificity ensures minimal cross-reactivity with mitochondrial AST (GOT2) or other aminotransferase isoforms.
Complete kit includes lyophilized standards, antibodies, and buffers for consistent and stable assay performance.
User-friendly colorimetric ELISA format enables rapid measurement via standard microplate readers without specialized equipment.
Process Relevance
Monitors cytoplasmic AST to assess liver function, metabolic pathways, and enzymatic biomarkers in human clinical research.
Application Stage
Clinical research, drug development, and preclinical biomarker evaluation in human samples.
Applications
Applicable for ELISA-based quantification in serum, plasma, tissue lysates, and body fluids, supporting ADME profiling studies.
Target
Cytoplasmic Aspartate Aminotransferase (AST1)
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, metabolic disease research, pharmacokinetics (ADME), toxicology studies, clinical diagnostics, enzyme activity profiling, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using colorimetric sandwich ELISA with high precision.
Assay Range
0.31 ng/ml - 20 ng/ml
LOD
0.19 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Cytoplasmic AST (GOT1) catalyzes the reversible transamination between aspartate and α-ketoglutarate, producing oxaloacetate and glutamate. Dysregulated AST1 activity is linked to liver injury, metabolic disorders, and cardiovascular risk, making it a key biomarker for biomedical research.
Pathways
AST1 participates in amino acid metabolism, the malate-aspartate shuttle, and cytoplasmic energy regulation. Upstream substrates include aspartate and α-ketoglutarate, while downstream effects influence the TCA cycle and nitrogen balance in hepatocytes.
For Research Use Only | Not For Clinical Use
Online Inquiry