T-Stimu™ Recombinant IE-1 Protein (CAR-TS-ZP1)

Datasheet

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

In contrast to peptides and unmodified proteins, T-Stimu™ proteins are processed and presented via functional antigen presenting cells (APC) through exogenous (MHC-II) and endogenous (MHC-I; cross-presentation) pathways, thus imitating natural infections.

In addition, the T-Stimu™ protein can produce more effective and HLA-independent stimulation of a broad subgroup of antigen-reactive effector cells (CD4 + and CD8 + T cells, NK and NKT-like cells).

Specifically, T-Stimu™ IE-1 recombinant protein is processed through the exogenous and endogenous processing pathways of APC, and epitopes are loaded on MHC-II and MHC-1 molecules through cross-display, thereby inducing the activation of antigen-specific effector cells (including CD4 + T cells, CD8 + T cells, NK cells and NKT-like cells).

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Specifications

Target
IE-1 (immediate-early protein 1)

Organism
Human cytomegalovirus (HCMV)

Application
• Activation of CMV reactive effector cell networks, including T helper cells (CD4 +), cytotoxic T cells (CD8 +), NK cells (CD56 +) and NKT-like cells (CD56 + CD3 +).
• Actively handled by APC to produce physiological and comprehensive stimulation.
• Stimulation and expansion of IE-1 specific effector cells for subsequent activation detection
• Induction of cytokine production in immunoassays.

Content
Stock solution of IE-1 (500 µl)

Capacity
For stimulation of up to 5 × 10*7cells

Format
Provided in a solution of sodium dihydrogen phosphate, sodium chloride and T-Stimu™ buffer.

Purity
> 90% as determined by SDS-PAGE analysis

Endotoxin Level
Low endotoxin

Storage
Store product protected from light at 2-8 °C

Shelf-Life
Expiry date is Lot specific (see label on vial)

Application Notes
Use IE-1 at a final dilution of 1:75. To stimulate 2×105 PBMC, prepare 50 µl of 1:25 dilution of IE-1 in the culture medium, and add 2 volumes of PBMC culture medium, the total volume of each well is 150 µl. If a higher concentration of antigen is required, use a dilution of at least 1:40 in the respective medium, because increased amounts of T-activation buffer may show cytotoxic effects.

Tag
Protein

Target

Target Introduction
Cytomegalovirus (CMV) is a virus with a seroprevalence rate of approximately 50% in the adult population, asymptomatic primary infection. Immediate Early Protein 1 (IE-1) is one of the earliest expressed viral gene products and plays a decisive role in acute infection and reactivation of the viral latency. Therefore, IE-1 antigen is a promising target for studying cellular immunity against CMV.

Entrez Gene ID
AAR31448.1

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

T-Stimu™ Recombinant IE-1 Protein (CAR-TS-ZP1)

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Specifications

Target

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