Green fluorescence protein (GFP) is a 27 kDa
protein derived from the jellyfish Aequorea victoria, which
emits green light (emission peak at a wavelength of 509 nm)
when excited by blue light (excitation peak at a wavelength of
395 nm). GFP has become an invaluable tool in cell biology
research, since its intrinsic fluorescence can be visualized in
living cells. GFP fluorescence is stable under fixation
conditions and suitable for a variety of applications. GFP has
been widely used as a reporter for gene expression, enabling
researchers to visualize and localize GFP-tagged proteins
within living cells without the need for chemical staining. Other
applications of GFP include assessment of protein protein
interactions through the yeast two hybrid system and
measurement of distance between proteins through
fluorescence energy transfer (FRET) protocols. GFP
technology has considerably contributed to a greater
understanding of cellular physiology. YFP differs from GFP
due to a mutation at T203Y; antibodies raised against fulllength
GFP should also detect YFP and other variants

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