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Anti-GFP (GFP200-208) TCR, pMSGV (TCR-L021)

The vector of anti-GFP T cell receptor (TCR) is constructed for the engineering of T cell to target GFP. The T cells are genetically modified through transduction with a retroviral vector expressing GFP-specific T cell receptor.

Details

  • Target
  • GFP
  • Epitope
  • GFP200-208
  • Format
  • Non-modified TCR
  • HLA
  • HLA-A2
  • Vector Name
  • pMSGV
  • Vector Length
  • 6700bp
  • Vector Type
  • retroviral vector
  • Antibody Host
  • Mouse

Target

  • Gene Name
  • Green fluorescence protein
  • Official Symbol
  • GFP
  • Synonyms
  • green fluorescent protein; GFP; RFP; BFP/GFP; TCR; T cell receptor; transgenic TCR; antigen-specific T cell receptors; Engineering T-cell receptors; recombinant t cell receptors; specific t cell receptor; modified t cell receptor; retroviral vector
  • Introduction
  • Green fluorescence protein (GFP) is a 27 kDa
    protein derived from the jellyfish Aequorea victoria, which
    emits green light (emission peak at a wavelength of 509 nm)
    when excited by blue light (excitation peak at a wavelength of
    395 nm). GFP has become an invaluable tool in cell biology
    research, since its intrinsic fluorescence can be visualized in
    living cells. GFP fluorescence is stable under fixation
    conditions and suitable for a variety of applications. GFP has
    been widely used as a reporter for gene expression, enabling
    researchers to visualize and localize GFP-tagged proteins
    within living cells without the need for chemical staining. Other
    applications of GFP include assessment of protein protein
    interactions through the yeast two hybrid system and
    measurement of distance between proteins through
    fluorescence energy transfer (FRET) protocols. GFP
    technology has considerably contributed to a greater
    understanding of cellular physiology. YFP differs from GFP
    due to a mutation at T203Y; antibodies raised against fulllength
    GFP should also detect YFP and other variants

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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