anti-GFP (aa200-208) TCR, pCDTCR1(TCR-L021Z)

The vector of anti-GFP T cell receptor (TCR) is constructed for the engineering of T cell to target GFP. The T cells are genetically modified through transduction with a retroviral vector expressing GFP-specific T cell receptor.


Details


Target
GFP
Epitope
GFP[200-208]
Format
Non-modified
HLA
HLA-A2
Vector Name
pCDTCR1
Vector Length
8kb
Vector Type
Lentiviral
Antibody Host
Mouse

Target


Gene Name
Green fluorescence protein
Official Symbol
GFP
Synonyms
green fluorescent protein; GFP; RFP; BFP/GFP; TCR; T cell receptor; transgenic TCR; antigen-specific T cell receptors; Engineering T-cell receptors; recombinant t cell receptors; specific t cell receptor; modified t cell receptor; retroviral vector
Introduction
Green fluorescence protein (GFP) is a 27 kDa
protein derived from the jellyfish Aequorea victoria, which
emits green light (emission peak at a wavelength of 509 nm)
when excited by blue light (excitation peak at a wavelength of
395 nm). GFP has become an invaluable tool in cell biology
research, since its intrinsic fluorescence can be visualized in
living cells. GFP fluorescence is stable under fixation
conditions and suitable for a variety of applications. GFP has
been widely used as a reporter for gene expression, enabling
researchers to visualize and localize GFP-tagged proteins
within living cells without the need for chemical staining. Other
applications of GFP include assessment of protein protein
interactions through the yeast two hybrid system and
measurement of distance between proteins through
fluorescence energy transfer (FRET) protocols. GFP
technology has considerably contributed to a greater
understanding of cellular physiology. YFP differs from GFP
due to a mutation at T203Y; antibodies raised against fulllength
GFP should also detect YFP and other variants

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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