The efficacy of many antibody-based therapies including antibody-drug conjugates (ADC), liposomal drugs, immunotoxins, and targeted gene delivery, rely on internalization process to confer their intended effects. Empowered by innovative techniques and rich experience in this field, scientists in Creative Biolabs has established a versatile internalization assessment platform. There are multiple assay formats available, from which you can always find the best fit for your candidate. In particular, we are happy to present our Magic™ whole fluorescent cell lines for internalization studies.
Introduction to Internalization
Generally speaking, there are 2 kinds of internalization formats: receptor-mediated and non-receptor-mediated. The former one is the most conventional internalization pattern. Upon binding with membrane receptors, the target molecule can translocate into the cytosol in endosomes, and subsequently undergoes a complex array of recycling / degradative pathways. This procedure is considered to correlate with many factors, such as target receptor, binding affinity, etc.
Internalizing antibodies have been shown to promote or inhibit cell growth, mainly relying on the epitope recognized. Inhibitory compounds can be used as a cure for cancer or for other conditions characterized by cell hyperproliferation, and for treating inflammation (anti-inflammatories). Stimulatory antibodies could be used for stimulating the growth of relevant cells like stem cells.
Fig. 1 The complex interply between GPCR signaling and internalization.1
Magic™ Whole Fluorescent Stable Cell Lines
We now offer a comprehensive set of stable transfected cell lines for high-throughput screening of internalizing compounds. These cell lines stably express high levels of target receptors (e.g. GPCRs, ion channels) fused with a fluorescent biosensor protein. These cell lines are designed to assay compounds or analyze stimuli for their ability to modulate receptor internalization by tracking cellular fluorescence intensity & location, which can make excellent tools for internalizing antibodies screening and validation. There are more than 20 ready-to-use stable cell lines available in Creative Biolabs for internalization assessment (as shown below):
| ADRB2 | BDKR1 | BDKR2 |
| CALCR | CB2 | CCR2 |
| CRHR2 | CXCR2 | DOR |
| DRD1 | DRD3 | DRD5 |
| FPRL1 | GLP2R | KOR |
| M1 | NK1R | NK3R |
| PAC1 (ADCYAP1R1) | S1P1R | SSTR2 |
| SSTR3 | SSTR5 |
As alternative, we are always happy to establish custom cell lines of any specific target upon request. Moreover, we also provide a variety of other stable cell lines for diverse in vitro function studies, e.g. GPCR biosensor cell lines for function screening. Our scientific team looks forward to leveraging our expertise and resources to help with all kinds of custom projects. Please feel free to contact us or directly sent us an inquiry.
Reference
For Research Use Only.
