Immunostimulatory receptors are emerging as promising targets for cancer immunotherapies. As a leading antibody service provider, Creative Biolabs has established a panel of assays for screening of safer and more efficient antibodies against various immune checkpoints, including both co-inhibitory and co-stimulatory molecules. OX40 is one of the co-stimulatory molecules that have been targeted for tumor treatment. Here, we present the OX40 assay as an attractive method for assessing the potency and mechanism of action of anti-OX40 antibodies.
OX40 (CD134), a member of the tumor necrosis factor receptor (TNFR) superfamily, is a co-stimulatory molecule. Structurally, this glycoprotein contains a cytoplasmic tail, a transmembrane domain, and an extracellular region. OX40 is predominantly expressed by T cells. It is transiently expressed by human CD4+ and CD8+ T cells following T cell receptor (TCR) stimulation. The ligand for OX40, OX40L (CD252), is classically expressed on antigen-presenting cells (APCs). Binding of OX40 to its ligand modulates T cell activation and T cell effector function. The OX40-OX40L pathway has been targeted in tumor immunotherapy. Anti-OX40 agonistic monoclonal antibodies and OX40L-Fc fusion proteins are currently tested in early phase cancer clinical trials. They have been reported to increase antitumor immunity and improve tumor-free survival. Besides, clinical trials with OX40 agonists are ongoing in patients with tumors, with both monotherapy and combination therapy included.
Fig. 1 The OX40L-OX40 axis stimulates the proliferation of effector Th1 and Th2 cells.1
To aid in the binding & interaction characterization of anti-immune checkpoint antibody, Creative Biolabs offers a broad and integrated portfolio of immune checkpoint interaction analysis services. Especially, we offer immune checkpoint-targeted reporter gene assays to investigate the potency and mechanism of action of your antibody candidates. Our assays cover the most popular immune checkpoints. These assays overcome the limitations of primary cell-based assays, including complex assay protocols, unqualified assay reagents, and dependent on donor primary cells.
Moreover, Creative Biolabs also offers professional immune checkpoint functional assays, providing measurements of T cell proliferation, T cell cytotoxicity, and cytokine release. Creative Biolabs is professional in designing custom assay protocols to meet all kinds of expectations and requirements. We are happy to work with you step by step so as to ensure that most satisfactory results can be acquired. If you are interested in our services, please contact us or send us an inquiry.
Reference
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