PD-1 Blockade Assay

Creative Biolabs is a first-class service provider focused on antibody functional assays. PD-1 blockade assay is an effective method which enables to measure the activity of antibody targeting PD-1 and to explore the mechanism of action of biologics designed to block PD-1. Aided by our experienced scientists and advanced technology platforms, we provide first-in-class PD-1 blockade assay to screen antibody candidates.

Introduction to PD-1/PD-L1

PD-1, also known as programmed cell death protein 1 or CD279 (cluster of differentiation 279), is a cell surface receptor that is a member of the immunoglobulin superfamily. It is expressed on T cells and pro-B cells. PD-L1 and PD-L2 are two ligands of PD-1. PD-L1 is expressed on almost all murine tumor cell lines, while PD-L2 is expressed mainly by DCs and a few tumor lines. PD-1 is an immune checkpoint that regulates the immune system’s response to the cell of the human body by down-regulating the immune system and promoting self-tolerance by suppressing T cell inflammatory activity. It has been reported that PD-1 is a key regulator against autoimmune diseases by promoting programmed cell death of antigen-specific T-cells in lymph nodes and reducing apoptosis of regulatory T cells (anti-inflammatory, suppressive T cells). Furthermore, PD-1 is also involved in the progression of multiple types of tumor. The interaction between PD-1 and PD-L1 has been shown to inhibit T lymphocyte proliferation, survival and effector functions, induce apoptosis of tumor-specific T cells, enhance the differentiation of CD4⁺ T cells into Foxp3⁺ regulatory T cells and the resistance of tumor cells to cytotoxic T lymphocyte (CTL) attack. Therefore, blocking the interaction of PD-1 and PD-L1 is a promising strategy for the treatment of the tumor. Some antibodies targeting the interaction have been trialed within the clinic for use in different types of cancer, such as non-small cell lung cancer.

Fig.1 PD-1/PD-L pathway and therapeutic targeting. (Long, 2017)

PD-1 Blockade Assay

PD-1 blockade assay is a bioluminescent cell-based assay that overcomes the limitations of current methods (such as complex assay protocols, unqualified assay reagents, and heterogeneous source of donor primary cells). This test can be used to measure the potency and stability of antibodies and other biologics targeting PD-1. Besides, it is also used to describe the mechanism of action of biologics designed to block the interaction of PD-1 and PD-L1. Creative Biolabs has accumulated extensive experience in antibody-receptor function assays. We are proud to offer a high-quality PD-1 blockade assay for global customers. Our services are characterized by:

• Excellent expert team for offering real-time technical guidance
• Advanced assay platform to promote the data output
• Timely data feedback to facilitate the process of your project

Popular Products

Creative Biolabs provides an extensive range of PD-1 bioassay kits and PD-1 cell lines designed to characterize PD-1 and support the development of novel therapeutics.

Customer Review

Creative Biolabs offers the high-quality PD-1 blockade assay which is useful for functional analysis of antibodies targeting PD-1. We are dedicated to facilitating the transformation of antibody drugs from preclinical to clinical. For more detailed information, please feel free to contact us or directly sent us an inquiry.

Reference

1. Long, J.; et al. PD-1/PD-L blockade in gastrointestinal cancers: lessons learned and the road toward precision immunotherapy. Journal of Hematology & Oncology. 2017, 10(1):146.
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