TIM3 Blockade Assay

As an acknowledged antibody functional analysis service provider, Creative Biolabs offers first-in-class services of TIM3 blockade assay to help our customers obtain reliable antibody functional analysis results, contributing to your research and projects successfully.

Introduction to TIM3

T-cell immunoglobulin and mucin-domain-containing-3 (TIM-3), also known as hepatitis A cellular receptor 2, belongs to the TIM family cell surface receptor proteins that share a similar structure, in which the extracellular region consists of membrane distal single variable immunoglobulin domain (IgV) and a glycosylated mucin domain of variable length located closer to the membrane. This protein is an immune checkpoint expressed by subtypes of lymphoid cells, such as lymphocytes Th1, Th17, Tc1, NK, as well as by myeloid cells. TIM-3 has reported being involved in the mediation of CD8⁺ T-cell exhaustion together with other inhibitory receptors including PD-1 and LAG-3. Furthermore, this protein is also a Th1-specific cell surface protein which regulates macrophage activation, suppresses Th1-mediated auto- and alloimmune responses, and enhances immunological tolerance. Studies have reported TIM-3 regulates immune tolerance in mouse models of infectious diseases, alloimmunity, autoimmunity, and tumor immunity. Therefore, TIM-3 seems to be a promising target for current immunotherapy.

Fig.1 Potential role of TIM-3 on macrophages.¹

TIM3 Blockade Assay

TIM3 blockade assay is a bioluminescent cell-based assay that overcomes the limitations of current methods (such as complex assay protocols, unqualified assay reagents, and independent of donor primary cells). This test can be used to measure the potency and stability of antibodies and other biologics targeting TIM3. Besides, it is also used to describe the mechanism of action (MOA) of biologics blocking TIM3. Creative Biolabs is proud to offer high-quality TIM3 blockade assay services for global customers. Our services are characterized by:

• Excellent expert team for offering real-time technical guidance
• Advanced assay platform to promote the data output
• Timely data feedback to facilitate the process of your project

Popular TIM3 Products

Our team at Creative Biolabs specializes in the field of immunology and has extensive experience in developing effective kits for studying LAG3-based therapeutic solutions. Our EasyMeasure™ TIM-3 Bioassay Kit (CAT#: AK-LX001) and EasyMeasure™ TIM-3 Bioassay Kit-5XBP (CAT#: AK-LX018) are reliable tools designed for accurate assessment of TIM3 blockade activity. In addition, we offer specialized cell lines PathSpecific™ Human TIM3 HEK293 Cell Line (CAT#: ZL-0724-WR14) expressing TIM3 to aid in the evaluation and development of anti-LAG3 antibody therapies.

Published Data

Background: Multiple myeloma (MM) is a cancer that originates in the plasma cells within the bone marrow. Although treatment options have improved, it continues to be a difficult disease to fully eradicate. One promising approach in cancer therapy is harnessing the power of natural killer (NK) cells, which have shown strong anti-tumor activity and potential in immunotherapy for various cancers, including MM.

Purpose: To investigate the impact of inhibiting the Tim-3 pathway on the ability of NK cells to target multiple myeloma (MM) in laboratory settings and live subjects, researchers utilized antibodies targeting Tim-3 and its ligands in this study.

Results: The findings indicate that targeting Tim-3 could be a promising approach for developing antibody- or NK cell-based immunotherapies against multiple myeloma.

Fig.2 Blocking Tim-3 improved the ability of NK cells to kill multiple myeloma cells in the laboratory.²

With the years of experience in the field of antibody functional assays, Creative Biolabs is specialized in offering the best TIM3 blockade assay service which is useful for functional analysis of antibodies targeting TIM3. For more detailed information, please feel free to contact us or directly sent us an inquiry.

Reference

1. Ranferi, O.G.; et al. TIM-3 regulates distinct functions in macrophages. Frontiers in Immunology. 2016, 7:229.
   Distributed under Open Access License CC BY 4.0, without modification.
2. Jiang, Wen, et al. "Tim-3 blockade elicits potent anti-multiple myeloma immunity of natural killer cells." Frontiers in Oncology 12 (2022): 739976.
   Distributed under Open Access License CC BY 4.0, without modification.

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