CytoFinal™ Human Lactate Dehydrogenase C (LDHC) ELISA Kit

CAT#: ITS-0326-WB115
Product Type: ELISA Kit
Species: Human
Target: Human Lactate Dehydrogenase C (LDHC)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitative ELISA kit for detecting human Lactate Dehydrogenase C (LDHC) in biological samples.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Human Lactate Dehydrogenase C (LDHC) ELISA Kit provides a sensitive and reliable method for quantifying LDHC protein levels in human-derived samples. Utilizing a sandwich ELISA format with HRP-based colorimetric detection, this assay enables accurate measurement of LDHC in serum, plasma, tissue lysates, and cell extracts for reproductive biology, cancer biomarker, and metabolic research.
Features
Highly specific sandwich ELISA format using optimized antibody pairs enables precise detection of human LDHC with minimal interference from related LDH isoforms.
Sensitive assay design and broad linear detection range allow accurate quantification of LDHC across multiple human biological sample types.
HRP-TMB colorimetric detection system produces stable absorbance signals compatible with standard microplate readers for efficient experimental analysis.
Comprehensive kit format including precoated microplates, standards, buffers, and detection reagents ensures streamlined operation and consistent experimental reproducibility.
Process Relevance
Measures LDHC expression to investigate reproductive metabolism and cancer-testis antigen activity in experimental studies.
Application Stage
Cancer research, reproductive biology studies, biomarker discovery, and translational biomedical research.
Applications
Quantification of human LDHC in serum, plasma, tissue lysates, and cultured cells for reproductive biology studies, cancer-testis antigen research, metabolic pathway analysis, ADME profiling studies, and biomarker investigation.
Target
Human Lactate Dehydrogenase C (LDHC)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Cancer biomarker research, reproductive biology, metabolic enzyme studies, pharmacokinetics (ADME), tumor metabolism, immunotherapy target discovery, developmental biology, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of LDHC protein concentration based on ELISA absorbance.
Assay Range
1.56 ng/ml - 100 ng/ml
LOD
0.94 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase C (LDHC), also known as the testis-specific LDH isoform, plays a crucial role in energy metabolism during spermatogenesis. It supports efficient ATP generation required for sperm development and motility. In addition, LDHC is classified as a cancer-testis antigen and is aberrantly expressed in several tumor types, making it an emerging biomarker and potential immunotherapy target.
Pathways
LDHC participates in glycolytic metabolism by catalyzing the reversible conversion between pyruvate and lactate while maintaining cellular NAD+/NADH balance. This reaction supports high energy demand in germ cells. In tumor biology, LDHC expression is linked to metabolic reprogramming pathways that promote enhanced glycolysis and cellular proliferation.
For Research Use Only | Not For Clinical Use
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