CytoFinal™ Mouse Tissue/Cell Specific Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit

CAT#: ITS-0326-WB71
Product Type: ELISA Kit
Species: Mouse
Target: Mouse alanine aminotransferase 1 (ALT1/GPT)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively detects mouse ALT1 protein levels in tissues and cells using a sensitive ELISA assay.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Mouse Tissue/Cell Specific Alanine Aminotransferase 1 / ALT (GPT) ELISA Kit provides a sensitive immunoassay for quantitative detection of ALT1 protein in mouse-derived biological samples. Utilizing a sandwich ELISA format with highly specific antibodies, the kit enables reliable measurement of ALT concentrations in tissue lysates and cultured cells, supporting mechanistic studies of liver injury and metabolic regulation in murine models.
Features
Mouse-specific antibody pair ensures selective recognition of ALT1 with minimal cross-reactivity against related aminotransferase enzymes in complex lysates.
Sensitive sandwich ELISA detection system allows reliable quantification of low-level ALT protein expression in mouse tissue and cellular samples.
Standard 96-well microplate assay format enables efficient parallel processing of multiple samples using widely available ELISA readers.
Carefully optimized reagents and calibration standards deliver reproducible quantitative measurements and consistent assay performance across independent experimental runs.
Process Relevance
Measures ALT protein levels to evaluate hepatocellular damage and metabolic enzyme regulation in mouse experimental models.
Application Stage
Preclinical research, mechanistic studies, toxicology evaluation, and metabolic disease modeling.
Applications
Quantitative measurement of ALT protein in mouse tissues and cultured cells for liver injury studies, metabolic pathway analysis, toxicology evaluation, and ADME profiling studies, preclinical biomarker research.
Target
Mouse alanine aminotransferase 1 (ALT1/GPT)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Hepatology, toxicology, metabolic disease research, pharmacology, pharmacokinetics (ADME), preclinical biomedical research, molecular metabolism, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement derived from absorbance signals generated in a sandwich ELISA assay.
Assay Range
0.156 ng/ml - 10 ng/ml
LOD
< 0.07 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Alanine aminotransferase 1 (ALT1) is a cytosolic enzyme that catalyzes the reversible transfer of an amino group from alanine to α-ketoglutarate. This reaction produces pyruvate and glutamate, linking amino acid metabolism with cellular energy pathways. In mice, changes in ALT levels are commonly used as indicators of hepatocellular injury and metabolic imbalance.
Pathways
ALT participates in the alanine cycle, which connects peripheral amino acid metabolism with hepatic glucose production. The resulting pyruvate enters gluconeogenesis or the tricarboxylic acid (TCA) cycle, integrating nitrogen metabolism with cellular energy generation.
For Research Use Only | Not For Clinical Use
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