CAR-T Cell Engineering Service

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

In addition to providing CAR construction service, Creative Biolabs is proficient in the development of diverse CAR-T cells, which have demonstrated their effectiveness in the promising field of adoptive CAR-T cell therapy for the treatment of a wide range of cancer types. Our team of expert scientists possesses extensive expertise in CAR technology and offers a comprehensive suite of solutions that cater to the diverse needs of researchers.

CAR-T Cell Types We Can Provide

Second-Generation CAR-T Cells
Second-generation CAR-T cells included two intracellular signaling domains, typically CD3ζ and costimulatory molecules such as CD28 or 4-1BB. These modifications significantly enhanced CAR-T cell functionality, leading to more promising clinical outcomes.
Third-Generation CAR-T Cells
The third-generation CAR-T cells extended the improvements further by incorporating multiple costimulatory domains, amplifying the T-cell response. These advanced constructs have demonstrated even greater success in various hematological and solid tumor malignancies.
Fourth-Generation CAR-T Cells
Fourth-generation CAR-T cells, also known as TRUCKs (T cells Redirected for Universal Cytokine-Mediated Killing), have been engineered to secrete cytokines or other therapeutic molecules, thereby enhancing their anti-tumor effects and creating a potential for universal CAR-T cell platforms.

CAR-T Cell Characterization

At Creative Biolabs, quality control is paramount. We conduct extensive in vitro and in vivo characterization to ensure the safety and efficacy of the CAR-T cells.

Phenotypic Analysis
We conduct extensive phenotypic analysis to evaluate the composition of CAR-T cell populations, including memory and effector subtypes. This information informs the choice of CAR construct and co-stimulatory domains for optimal therapeutic results.
Functional Assays
Functional assays, such as cytotoxicity and cytokine release assays, are crucial in assessing CAR-T cell activity. Our rigorous testing protocols guarantee that the engineered cells effectively target and destroy tumor cells.
Purity Assessment
To meet the highest safety standards, we perform purity assessments, ensuring that the final product contains minimal contaminants and is free from any undesired cells.

General Workflow

Our comprehensive workflow for CAR-T cell engineering ensures that every step is meticulously executed, from vector design to final product delivery.

General Workflow

Our Advantages

Cutting-edge Technology in CAR-T Cell Engineering
We can precisely modify the CAR-T cell genome to improve its functionality by utilizing CRISPR-Cas9 technology. This technique enables the deletion of inhibitory receptors or the insertion of additional target recognition domains, further enhancing the therapeutic potential of CAR-T cells.
Advanced Gene Delivery Methods
Creative Biolabs employs cutting-edge gene delivery methods, including viral vectors like lentiviruses and retroviruses, as well as non-viral approaches like transposon-based systems, to efficiently introduce CAR constructs into T cells. Our expertise in viral vector design and optimization ensures the generation of potent CAR-T cells.
Customized CAR Design and Optimization
Our team specializes in CAR design, tailoring these receptors to the specific antigen of interest. We integrate multiple signaling domains, select suitable costimulatory molecules, and optimize spacer regions to enhance CAR-T cell efficacy and minimize off-target effects.

Contact us to learn more about our CAR-T Cell Engineering Service and explore the possibilities it holds for your research or clinical applications.