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NR4A Knock Out CAR-T Cell Development with CRISPR Technology

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

As one of the current most popular immunotherapies, CAR-T therapy shows its strength by boosting the patients’ own immune system to destroy cancer. Creative Biolabs has accumulated extensive experience in the discovery and development of optimized CAR-T therapies. Leverage the expertise of our experienced scientists to expedite and innovate your therapeutic CAR-T projects, we are inspired by NR4A target and are dedicated to providing NR4A knock out CAR-T cell development services.

Introduction of the Novel Target - NR4A

Recently, NR4A was identified as a key mediator of T cell dysfunction through genomic analysis, and also, NR4A transcription factors play the inhibitory role in CAR-T cell function in solid tumors. These two studies indicate that NR4A may be one of the potential targets to improve the CAR-T efficacy in solid tumors. Chen Dong’s team used an in vitro T cell tolerance induction system to characterize genome-wide epigenetic and gene expression levels in tolerant T cell. Finally, they found that NR4A1 shows high expression levels in tolerant T cells. Moreover, NR4A1 deletion overcomes T cell tolerance and promote effector function, as well as enhancing anti-tumor and chronic virus immunity (see Fig.1 left). As a key regulator in the T cell dysfunction, NR4A1 is a potential target for tumor immunotherapy.

(left) Disruption of NR4A1 prevents T cell exhaustion caused by tumor and viral infection. (Liu, 2019); (right) NR4A-deficient CAR TILs promote tumor regression and prolong survival. (Chen, 2019)

Fig.1 (left) Disruption of NR4A1 prevents T cell exhaustion caused by tumor and viral infection. (Liu, 2019); (right) NR4A-deficient CAR TILs promote tumor regression and prolong survival. (Chen, 2019)

Joyce Chen’s team found that NR4A triple knockout (NR4A1, NR4A2, NR4A3) CAR-T cells promoted tumor killing and improved the survival of tumor-bearing mice (see Fig.1 right). This indicates that NR4A inhibition can be as a promising strategy for cancer immunotherapy.

Our NR4A Knock Out CAR-T Cell Development Services

Over a decade of extensive experience in one-stop CAR-T cell therapy development, Creative Biolabs has established a series of solutions including Biomarker Identification & Selection, scFv Generation, CAR Design & Construction, CAR-T Gene Packaging & Delivery, In Vitro Assay Services, Preclinical In Vivo Assays, etc. Recently, our scientists developed cutting-edge CRISPR-Cas9 technology to edit CAR-T cells. Our CRISPR/Cas9 solution will help our clients to develop the highly efficient engineered CAR-T cells with improved anti-tumor efficacy. Based on our one-stop CAR-T cell development services and CRISPR/Cas9 technology, we are dedicated to providing NR4A knock out CAR-T cell development services to advance your CAR-T cell therapy to treat solid tumors.

NR4A Knock Out CAR-T Cell Development with CRISPR Technology

Earning the Trust of Our Clients Everywhere, Every Day

-Leverage the expertise of our senior scientists to expedite and innovate your CAR-T therapy-

Highlight Features

  • Comprehensive: a series of advanced platforms,
  • Guaranteed quality: based on our advanced facilities and technologies,
  • End-to-end solutions: a complete solution from molecular services to preclinical assays,
  • Custom service: customized service to ensure your flexible choice in an efficient manner.

As a leading CAR-T therapy development provider, Creative Biolabs is committed to guiding our clients in an end-to-end manner to ensure the optimal strategy and fit your expected features within budget and timeline. If you are interested in NR4A to knock out CAR-T cell development, please contact us for more information and a detailed quote.

References

  1. Liu, Xindong, et al. "Genome-wide analysis identifies NR4A1 as a key mediator of T cell dysfunction." Nature (2019): 1.
  2. Chen, Joyce, et al. "NR4A transcription factors limit CAR T cell function in solid tumours." Nature (2019): 1.

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

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