CAR-γδ T Cell Subsets Sorting Services

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

CAR-T therapies are under development using γδ T cells. Creative Biolabs is committed to offering one-stop CAR-γδ T cell services. Based on our advanced facilities and technologies, we provide γδ T cell subsets sorting services to develop personalized γδ T-cell-based therapies.

Introduction

γδ T cells are a heterogeneous type of innate-like or unconventional T cell. Ever increasing complexity in both phenotype and function is being ascribed to γδ cell subsets from various tissues and locations. Human γδ T cells can be classified into different groups based on the expression of TCRγ chains or TCRδ chains, and they can be further classified by the expression of different CD molecules. Different γδ T-cell exert diverse actions in distinct pathological types of cancer. Additional studies should be carried out on human γδ T-cell subsets to develop personalized γδ T-cell-based therapies.

Based on the TCR structure, human γδ T cells can be divided into four main populations based on TCR δ chain expression (δ1, δ2, δ3, δ5). Vδ1, Vδ2, Vδ3, and Vγ2, Vγ3, Vγ4, Vγ5, Vγ8, Vγ9, and Vγ11 are the most frequently gene segments used in rearrangement of δ and γ chains, respectively. The majority of the tissue-associated γδ T cells bear the Vδ1 TCR with Vδ3 and Vδ5 making up minor populations. Vδ2 T cells constitute the majority of blood γδ T cells. Vγ9Vδ2 T cells are the predominant γδ T cell type in human peripheral blood, and make about 60%-95%.
γδ T cells bear the CD3 antigen, but most lack CD4 and CD8 and are therefore termed double-negative T cells, although a small fraction can also express the αβ TCR on their surface. Four subsets of human γδ T-cells have been identified based upon the expression of the CD45 and CD27 surface markers:naïve(CD45RA+, CD27+), central memory (CM: CD45RA-, CD27+), effector memory (EM: CD45RA-, CD27-), and terminally differentiated (EMRA: CD45RA+, CD27-).
Similar to conventional T lymphocytes, γδ T cells can be functionally divided into subsets, e.g., Th1-, Th2-, Th9-, Th17-, Tfh, regulatory T cell (Treg)- and antigen-presenting cell (APC)-like γδ T cells

Fig.1 Major populations of human γδ T cells. (Xu, 2020)

γδ T cell subsets can be segregated on the basis of surface expression levels of receptors. Using flow cytometry, we have the ability to identify a subset of γδ T cells. We also provide available commercially antibodies for fluorescence-activated cell sorting (FACS) including anti-TCRγδ, anti-Vδ1, anti-Vδ2, anti-Vδ3 and isotype controls. Combined with other markers including CD56/CD28/CD5/CD3/CD16/CD8/CD27/CD45RA, etc. we can quantify and characterize γδ T cell and their subsets. For CAR-γδ T cell therapy, we can sort subsets of γδ T cells for specific CAR-γδ T research.

Backed by a team of highly skilled and trained biologists and drug discovery scientists, Creative Biolabs focuses on developing CAR-γδ T cell-based therapies against cancer. We provide γδ T cell subsets sorting services to meet your custom CAR-γδ T cell-based therapies. If you are interested in our services, please feel free to contact us.

Reference

Zarobkiewicz, M.K.; et al.γδ T lymphocytes in asthma: a complicated picture. Archivum Immunologiae et Therapiae Experimentalis, 2021, 69(1): 1-15.