CellRapeutics™ NKG2D-based Universal CAR Engineering Service Using TIM Molecule

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

NKG2D-based Immunotherapy with Prospect

NKG2D and its ligands play a variety of roles in tumor immunosurveillance. As an activating immunoreceptor, NKG2D plays an important function in anti-tumor immunity by recognizing tumor cells and mediating anti-tumor immune responses. The ligands of NKG2D are highly expressed on several malignant cells and are usually absent in healthy tissue, making it a potential target candidate for immunotherapy.

Fig.1 NKG2D receptor and its ligand.¹

CellRapeutics™ NKG2D-based Universal CAR Engineering Service Using TIM Molecule

Based on the fully-studied function of NKG2D-NKGDL in tumor immunosurveillance, Creative Biolabs has launched a custom CellRapeutics™ NKG2D-based universal CAR engineering service. Our CAR construct containing human NKG2D full-length extracellular domain that act as extracellular recognition domain and transmembrane domain, fusing into the intracellular signaling domain of CD3ς stabilized by naturally expressed DAP10. In addition, the NKG2D-CAR constructs contain a TCR Inhibitory molecule (TIM) to interfere with endogenous TCR signaling thought to contribute to GvHD. With this strategy, it is expected to enhance the function of CAR-T in the tumor microenvironment and induce a more durable anti-tumor response, thus improving the efficacy of CAR-T cells in patients.

Fig.2 Overview of NKG2D-CAR T cell therapy.²

Process diagram

Creative Biolabs provides a one-stop NKG2D-based universal CAR engineering service covering from CAR design to function validation to meet global customers' diverse needs.

Fig.3 The workflow of our CellRapeutics™ NKG2D-based Universal CAR Engineering Service. (Creative Biolabs)

Our Highlights

Optimized CAR Design & Construction

Powered by our years of professional experience in the CAR-T field, we offer customized services to achieve optimized CAR design & construction to meet the various needs of global customers.

Reliable and Reputable Experiment Systemn

Our experimental system has multiple strict quality control systems and is continually being optimized according to technological advances. Together with straightforward experimental procedures, we are fully confident that we can deliver desirable results for global customers.

Comprehensive Services

We provide one-stop NKG2D-based universal CAR engineering services covering from target selection and design optimization, to in vitro and in vivo CAR-T functional validation.

Frequently Asked Questions

Q1: What are the potential pitfalls to be considered in the NKG2D-based CAR engineering development?

A1: Regarding the NKG2D-based CAR-T development, there are some potential items that need to be taken into account. As the presence of NKG2D ligands doesn't always correlate with an enhanced anti-cancer cytolytic immune response, NKG2D-mediated tumor immune monitoring has considerable selection pressure on tumor survival. Therefore, some human tumor types shed NKG2DL from their cell surface to evade the immune response, resulting in a high levels of soluble NKG2DL. The binding of soluble NKG2D ligands leads to systemic NKG2D desensitization in effector cells and anti-tumor function damage. Thus, despite the NKG2D receptor may be a promising treatment target for cancer therapy, it should consider the high levels of soluble NKG2DL may damage immune cell responsiveness by down-regulating the NKG2D receptor.

Work with Creative Biolabs

For more details about our CellRapeutics™ NKG2D-based Universal CAR Engineering Service, please feel free to contact us. We will be pleased to receive your inquiry. Sincerely look forward to cooperating with global customers.

References

Duan, S.; et al. Natural killer group 2D receptor and its ligands in cancer immune escape. Mol Cancer. 2019, 18(1):29.
Curio, S.; et al. A summary of current NKG2D-based CAR clinical trials. Immunother Adv. 2021, 1(1):ltab018.