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Cytokine Release Assay

In recent years, T-cell therapy has yielded impressive clinical results in patients who had previously failed to standard treatments. Adoptively transferred T cells can kill antigen-expressing tumor cells and thereby provide a direct cytolytic effector function. Several TCR gene-modified T-cell trials have shown successes. Adoptively transferred T cells not only can directly kill tumor cells to enable fast tumor reduction but also can initiate late adaptive responses by other compartments of the immune system.

Creative Biolabs is a professional service provider in the field of T-cell receptors (TCRs) development and analysis, and now offers cytokines release assay service to help customer assess the function of their TCR-modified T cells by determining the cytokines releasing ability, in detail, detecting the quantity and types of cytokines secreted by those engineered T cells.

Principle of Cytokine Release

Cytokine Release Assay

TCRs on T-cell surfaces can specifically recognize peptide antigens presented by APCs, leading to the activation of T-cells and a series of functional outcomes such as cytokine production. The release of cytokines by T cells defines a significant part of their functional activity and their ability to produce multiple cytokines has been associated with beneficial immune responses. With the increasing of TCR gene-modified T-cell research and development, evaluating the cytokines release ability of TCR-modified T cells can be quite important for monitoring the function and efficiency.

How do we perform cytokine release assay?

Creative Biolabs commonly provides our clients the measurement methods including enzyme-linked immunospot assay (ELISpot), intracellular cytokine staining assay (ICS), and flow cytometry, but not limited to them. In response to viruses or tumor antigens, those successfully modified T cells can secrete higher levels of cytokines, such as IFN-γ, IL-12, and GM-CSF. Therefore, Creative Biolabs will focus on these kinds of cytokines and select appropriate methods for customers to analyze these released cytokines.

With access to various commercial ELISA assay kits and multiple analysis technology and instruments, Creative Biolabs is committed to offering top-quality cytokines release assay to meet every customer’s requirements. For assay projects, Creative Biolabs guarantee to offer a comprehensive analysis report with accurate assay data and all the other information required.

Illustration of the ELISPOT assay.

Fig.2 Illustration of the ELISPOT assay.

Features of CBL’s Cytokine Release Assay

Cytokine Release Assay-2

  • Developed in accordance with FDA recommendations for preclinical testing of biologics
  • Determination of 10 cytokines/chemokines: IL-2, IL-6, IL-8, IL-10, IL-1β, IL-12p40, IL-17A, IFNγ, MIP-1α and TNF-α
  • Choose any of our cryopreserved PBMC donors or custom tests using non-human PBMC
  • Appropriate controls provide air drying, wet adhesion or soluble antibody presentation methods
  • Incubation time is 24 or 48 hours
  • PBMC proliferation test on request
  • Additional markers can be added upon request

Creative Biolabs can enable you to free up your time for core work and projects. Our service can be designed to meet your special needs if you have any requirements. To discuss your demands or to request a proposal, please contact us by info@creative-biolabs.com.

All services and products are only for lab research use, not for any clinical diagnosis or treatment.

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