Cytokine Release Assay

Based on the outstanding expertise and rich experience, Creative Biolabs has specially developed a system approach of TCR generation and optimization for TCR development. Particularly, we offer cytokines release assay service to help customers assess the function of their TCR-modified T cells by determining the cytokines releasing ability.

In recent years, T-cell therapy has yielded impressive clinical results in patients who had previously failed to standard treatments. Adoptively transferred T cells have the capacity to kill antigen-expressing tumor cells and thereby provide a direct cytolytic effector function. Several TCR gene-modified T-cell trials have shown successes. Adoptively transferred T cells not only can directly kill tumor cells to enable fast tumor reduction, but also can initiate late adaptive responses by other compartments of the immune system. TCRs on T-cell surfaces can specifically recognize peptide antigens presented by APCs, leading to the activation of T-cells and a series of functional outcomes such as cytokine production. The release of cytokines by T cells defines a significant part of their functional activity and their ability to produce multiple cytokines has been associated with beneficial immune responses. With the increasing of TCR gene-modified T-cell research and development, evaluating the cytokines release ability of TCR-modified T cells can be quite important for monitoring the function and efficiency.

Creative Biolabs commonly provides our clients the measurement methods including enzyme-linked immunospot assay (ELISpot), intracellular cytokine staining assay (ICS), and flow cytometry, but not limited to them. In response to viruses or tumor antigens, those successfully modified T cells can secrete higher levels of cytokines, such as IFN-γ, IL-12 and GM-CSF. Therefore, Creative Biolabs will focus on these kinds of cytokines and select appropriate methods for customers to analyze these released cytokines.

Fig.1 Illustration of the ELISPOT assay

Creative Biolabs has the capability to enable you to free up your time for core work and project. Our service can be designed to meet your special needs if you have any requirements. If you are interested in our service, please contact us by E-mail and our team will get back to you as soon as possible.