All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
RNA interference (RNAi) is an excellent technology for functional gene silencing. To overcome the challenges in CAR-T therapy, genetic modification of CAR T cells by RNAi is a promising strategy. As the leader in CAR-T therapies, Creative Biolabs combines the CAR-T platform with RNAi-based gene silence technologies to develop next-generation strategies for advances in CAR-T therapy. We carry out a novel approach in which a miRNA or shRNA cassette is integrated into CAR-expressing retroviral vectors to generate an optimal therapeutic T cell phenotype.
Fig.1 Example of blockage of PD-1 in CAR-T cells by shRNA technologies.1
We have developed the following novel strategies to modulate and knockdown gene expression in our CAR T-cell:
Silence™ CAR construct is an all-in-one vector containing the silencer (shRNAs or miRNAs), the CAR, the cell selection marker, and therapeutic add-ons.
Fig.2 All-in-one Silence™ CAR vector.
At Creative Biolabs, we provide one-stop Silence™ CAR-T services covering the entire Silence™ CAR-T therapy development process. We evaluate the expression of silence target genes by PCR, western blot, and FC. We also provide a comprehensive set of in vitro and in vivo functional assays to meet your academic or clinical purposes. Feel free to contact us to customize services to suit your research needs.
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