T Cell Persistence Test Service

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T Cell Persistence in TCR-T Therapy

In the context of TCR-T therapy, T cell persistence refers to the ability of TCR-T cells to persist in the body after infusion. The persistence of TCR-T cells is important for their efficacy, as they need to remain in the body in sufficient numbers to continue targeting and killing cancer cells, leading to long-term antitumor activity. Good persistence of TCR-T cells can improve the effective antitumor response. Several factors can influence TCR-T cell persistence, including TCR-T cell designs and manufacturing processes, the type of TCR-T cell, the type and stage of the cancer being treated, and the patient's immune system.

At Creative Biolabs, we provide T-cell persistence test services that can be used to detect TCR-T cell persistence in vivo. Our experts offer several methods to monitor circulating TCR T-cells into blood samples after treatment.

Our Services

It is essential to understand and enhance TCR-T cell persistence thus improving the efficacy and durability of TCR-T cell therapies in treating cancer. T cell persistence test involves the detection and quantification of TCR T cells in blood samples. At Creative Biolabs, we monitor TCR T-cell persistence over time with several techniques, including flow cytometry, qPCR, and ddPCR. By combining multiple techniques, we aim to provide comprehensive information on TCR-T cell persistence and function, which is crucial for the optimization of TCR-T cell therapies. The following are some reliable methods of tracking TCR T cell numbers.

Fig.1 T Cell Persistence Tests.

Flow cytometry

Flow cytometry enables the identification of surface epitopes on live TCR T-cells. This method involves staining the TCR-T cells with fluorescent antibodies specific to the TCR molecule and other cell surface markers. The stained cells are then analyzed using flow cytometry to quantify the number of TCR-T cells present in a sample.

qPCR

qPCR is the gold standard for the detection and quantification of the integrated TCR transgene sequence in TCR-T cells. Monitoring TCR-T expansion/persistence through our qPCR assay gives you real-time data on the TCR-T therapy.

ddPCR

ddPCR is a powerful tool that can be used to determine the absolute number of TCR-T cells present in a blood sample. It enables the monitoring of TCR T-cell kinetics with high precision and sensitivity.

Support Data

Here are some representative data for T-cell persistence tests.

Case 1: Analysis of CAR-T Cell Persistence by ddPCR

Fig.4 CAR-T cell persistence with time was assessed by ddPCR. (Wittibschlager, et al., 2023) Fig.2 CAR-T cell persistence with time was assessed by ddPCR.¹

Reference

Wittibschlager, Valerie et al. “CAR T-Cell Persistence Correlates with Improved Outcome in Patients with B-Cell Lymphoma.” International journal of molecular sciences vol. 24,6 5688. 16 Mar. Distributed under Open Access license CC BY 4.0, without modification.

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

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