FASLG

Creative Biolabs provides a diverse range of anti-FASLG CAR products designed for the specific and effective targeting of cancers expressing FASLG. Our CAR products undergo rigorous quality control procedures to ensure their safety and effectiveness.

Background

Fas ligand (FASLG), also known as CD95L or CD178, is a transmembrane protein that is primarily expressed on activated T cells and natural killer (NK) cells. FASLG plays a crucial role in apoptosis (programmed cell death) through its interaction with the Fas receptor. By modulating the FASLG pathway, it is possible to selectively induce cell death in cancer cells while sparing normal cells or to regulate immune responses in autoimmune diseases.

Dysregulation of FASLG signaling has been implicated in various diseases, including autoimmune disorders and certain types of cancer. Targeting FASLG in therapy holds promise for the development of novel treatment strategies with the potential to improve outcomes for patients with various diseases. Therapeutic approaches targeting FASLG may include the use of monoclonal antibodies, small molecule inhibitors, or gene therapy to either inhibit or enhance FASLG signaling based on the specific disease context.

Fig.1 Schematic of FAS-FASLG pathway.¹

Associated Disease

• Systemic lupus erythematosus
• Colorectal cancer
• Breast cancers
• Ovarian cancer

Anti-FASLG CAR-T Services

Anti-FASLG CAR-T In Vitro Assays

• Anti-FASLG CAR-T Expression Test

We offer anti-FASLG CAR-T expression tests that are used to assess the expression and functionality of anti-FASLG CAR-T cells. The test typically involves techniques such as Western blotting (WB) and flow cytometry to analyze the expression levels of the CAR construct in the modified T cells.

• Anti-FASLG CAR-T Proliferation Test

CAR-T proliferation assays and techniques are utilized to measure the growth and expansion of CAR-T cells in cell culture. Our experts have rich experience in analyzing the proliferation kinetics of CAR-T cells. We provide anti-FASLG CAR-T proliferation test services that typically involve assessing the expansion and proliferation of CAR-T cells in response to specific stimuli or treatments.

• Anti-FASLG CAR-T Cytokine Release Test

Cytokine release tests are important for monitoring the activity and potential side effects of CAR-T cell therapy, as excessive cytokine release (known as cytokine release syndrome) can lead to serious adverse events in patients. At Creative Biolabs, we provide some common types of cytokine release tests such as ELISA, flow cytometry-based assays, and cytometric bead arrays that allow for the simultaneous detection and quantification of multiple cytokines in a single sample.

• Anti-FASLG CAR-T In Vitro Cytotoxicity Assay

Our team of experts offers comprehensive CAR-T cytotoxicity testing services, tailored to meet the specific needs of our clients. We offer several different types of in vitro CAR-T cell cytotoxicity assays such as LDH release assay, Cr-51 release assay, and luminescence-based cytotoxicity assay.

Anti-FASLG CAR-T In Vivo Assays

By integrating diverse methods and techniques, Creative Biolabs is able to comprehensively evaluate the anti-tumor efficacy of candidates or therapies in a physiologically relevant context. We provide a variety of in vivo assays including anti-tumor efficacy studies, toxicity assessments, and CAR-T persistence studies.

Reference

1. Khosroabadi, Ghazaleh, and Saghar Yousefnia. "Mechanistic and Diagnostic Roles of Kallikrein Related Peptidases 2 (FASLG) in Prostate Cancer." Personalized Medicine Journal 8.28 (2023): 5-12.