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TCR Clonality Assessment Service

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The field of immunology has undergone a paradigm shift with the advent of immunotherapy and personalized medicine. T-cell receptor (TCR) clonality assessment has emerged as a cornerstone technology for decoding adaptive immune responses, tracking disease trajectories, and evaluating therapeutic efficacy. However, researchers face significant challenges in capturing the full spectrum of TCR repertoire diversity, detecting minimal residual disease with high sensitivity, and translating complex genomic data into clinically actionable insights. At Creative Biolabs, we bridge this gap with our cutting-edge TCR Clonality Assessment Service, leveraging over 20 years of biological expertise to deliver precise, reliable, and interpretable data that drives innovation in oncology, autoimmunity, and infectious disease research.

Fig.1 Schematic of the nonstringent TCR cloning method^1,2

At Creative Biolabs, we offer comprehensive TCR clonality analysis solutions built upon a multidimensional cutting-edge technology platform, specifically designed for your high-end immunology research. From precise protocol design to deep data insights, our team remains your trusted research partner, delivering reliable, scalable, and highly interpretable data to accelerate your entire immunotherapy and disease mechanism research projects.

Why Choose Creative Biolabs?

Selecting the right partner for TCR clonality assessment is pivotal to the success of your projects. Creative Biolabs stands out through its unwavering commitment to excellence, backed by over 20 years of industry leadership.

Unmatched Expertise

Our team of PhD-level biologists and bioinformaticians brings deep knowledge in immunology and genomics, ensuring scientifically sound interpretations and recommendations

Superior Sensitivity and Specificity

With optimized wet-lab protocols and computational algorithms, we achieve detection limits as low as 0.001% for rare clonotypes, minimizing false positives and negatives

Rapid Turnaround and Scalability

Leveraging automated workflows, we handle projects of any size—from pilot studies to large cohorts—without compromising on quality or timelines

Dedicated Support

From project design to data delivery, our customer success team provides personalized guidance, helping you maximize the value of your investment.

Service Process

We have streamlined our service process to ensure efficiency, transparency, and client satisfaction. Below is an overview of the key steps involved:

01Consultation and Project Design

We begin by understanding your objectives, sample types, and experimental design. Our experts collaborate with you to tailor the service to your specific needs

02Sample Submission and Quality Control

Clients submit samples (e.g., blood, tissue, or FFPE blocks), which undergo rigorous QC checks for DNA/RNA integrity and concentration

03Library Preparation and Sequencing

Using optimized kits, we construct sequencing libraries and perform high-coverage NGS on Illumina or similar platforms

04Report Delivery and Follow-Up

You receive a detailed report via a secure portal, followed by a debriefing session to discuss findings and potential next steps

Technology and Innovation Platform

Our TCR clonality assessment service is not merely a collection of processes, but is built upon an integrated platform that deeply integrates cutting-edge wet lab techniques, proprietary bioinformatics algorithms, and big data insights. This ensures every data point you receive carries the depth and reliability of our technology.

Patented Primer Design & Multiplex PCR System

Comprehensive Unbiased Coverage: Our optimized multiplex PCR primer set efficiently amplifies the full-length CDR3 regions of both TCRα and TCRβ chains, ensuring comprehensive, unbiased capture of the T cell repertoire and minimizing data bias from amplification preferences.
Exceptional Sensitivity: Even with extremely low starting sample quantities, our patented system maintains high sensitivity, making it a powerful tool for tumor-infiltrating lymphocyte (TIL) research.

Dedicated Bioinformatics Cloud Platform

We understand raw data alone does not yield insights. Therefore, every client gains access to our proprietary bioinformatics cloud platform:

Interactive Visualization: Generate professional charts—including clonal distribution maps, dynamic evolutionary trajectories, and diversity index comparisons—via drag-and-drop operations without programming knowledge.

Instant Data Mining: Supports real-time filtering and querying of specific CDR3 sequences or V/J gene usage patterns, helping you rapidly pinpoint target clones.

Internal TCR Sequence Reference Database

Leveraging years of experience processing hundreds of thousands of samples, we have built and continuously optimized an extensive internal TCR sequence reference database.

Efficient Annotation: This database enables faster and more accurate annotation of gene segments (V, D, J) for clonal types obtained through sequencing.
Background Noise Filtering: Helps us effectively identify and filter out non-specific amplification products and technical background noise commonly encountered during sequencing, ensuring every clon reported to you holds genuine biological significance.

Services Offered

Our TCR Clonality Assessment Service offers a comprehensive suite of solutions tailored to meet the diverse needs of academic institutions, pharmaceutical companies, and clinical laboratories. Each component is meticulously crafted to ensure accuracy and scalability.

High-Throughput TCR Sequencing

Utilizing next-generation sequencing (NGS) platforms, we deliver deep profiling of TCR beta (TCRB) and TCR alpha (TCRA) chains. This includes full-length sequencing to capture complete CDR3 regions, enabling detailed clonotype identification and quantification.

Advanced Bioinformatics Analysis

Our proprietary pipelines perform robust data processing, including error correction, clonotype assembly, and diversity metrics calculation. We also offer comparative analysis across samples to track clonal dynamics over time or between conditions.

Customized Reporting and Visualization

Clients receive intuitive reports featuring clonotype frequency distributions, Shannon diversity indices, and phylogenetic trees. Interactive dashboards allow for easy exploration of data, facilitating hypothesis generation and decision-making.

Integration with Multi-Omics Data

For a holistic view, we can correlate TCR clonality data with transcriptomic, genomic, or proteomic datasets, providing insights into immune microenvironment interactions.

Applications

TCR clonality assessment has broad applicability across multiple domains, enabling breakthroughs in research and clinical practice:

Cancer Immunotherapy
Monitor T-cell responses to checkpoint inhibitors, CAR-T therapies, or vaccines; identify predictive biomarkers for treatment efficacy.

Autoimmune and Inflammatory Diseases
Characterize aberrant T-cell expansions in conditions like rheumatoid arthritis or multiple sclerosis to uncover pathogenic mechanisms.

Infectious Disease Research
Track antigen-specific T-cell repertoires during infections (e.g., HIV, COVID-19) or vaccination campaigns.

Transplant Medicine
Assess immune reconstitution and detect graft-versus-host disease-related clonal expansions in hematopoietic stem cell transplant recipients.

Drug Development
Support preclinical and clinical trials by evaluating immunogenicity and safety profiles of novel therapeutics

Technology Comparison

Feature	Standard Service	Premium Service
Sequencing Depth	50,000 reads/sample	500,000+ reads/sample
Clonotype Detection Limit	0.1%	0.001%
Analysis Complexity	Basic metrics	Advanced statistics & multi-omics integration
Turnaround Time	3-4 weeks	2-3 weeks
Expert Consultation	Standard report	Dedicated scientist support

Client Testimonials

Hear from researchers and industry leaders who have leveraged our service to achieve their goals:

"Creative Biolabs's TCR clonality analysis service has delivered breakthrough progress in our tumor immunology research. Its exceptional data quality enabled us to precisely track dynamic changes in T-cell clones before and after treatment, providing critical evidence for understanding resistance mechanisms."— Sarah Lin, Chief Researcher, Immunology Institute, Leading University
"During the development of novel cell therapy products, Creative Biolabs's in-depth TCR lineage analysis proved indispensable. Their technical team's multi-omics integration of data provided unique insights for optimizing our product design."— John Keller, Technical Director, Biotechnology Company
"As researchers focused on autoimmune diseases, Creative Biolabs's services enabled us to precisely identify pathogenic T cell clones. Their specialized bioinformatics analysis platform transformed complex data into intuitive and insightful visualizations."— Dr. Emily Roberts, Project Lead, Medical Research Center
"In our vaccine development project, we needed to evaluate the persistence of T-cell immune responses. Creative Biolabs's end-to-end solution—from experimental design to final reporting—demonstrated exceptional professionalism and reliability."— Zhao Min, Senior Scientist, Vaccine R&D Department, Pharmaceutical Company
"We previously submitted samples to multiple testing institutions for comparison. Creative Biolabs's data demonstrated the most outstanding accuracy and reproducibility in clonal quantification. This provides reliable assurance for our fundamental research."— Chen Hua, Postdoctoral Researcher at a Research Institute
"Creative Biolabs's technical support team is impressive. During our deep data mining, they provided professional and timely guidance, helping us uncover critical findings that might otherwise have been overlooked."— Sun Yue, Bioinformatician at a Gene Therapy Company
"We feel completely confident entrusting clinical samples to Creative Biolabs. Their rigorous quality control system and standardized sample management protocols ensure optimal testing results for every precious specimen."—Zhou Tao, Laboratory Director at a Clinical Testing Center

Frequently Asked Questions

What sample types are supported?

We accept peripheral blood mononuclear cells (PBMCs), fresh or frozen tissue, FFPE blocks, and extracted DNA/RNA. All sample types undergo rigorous nucleic acid quality and concentration validation to ensure data reliability.

What is the core technological principle behind this service?

Our service is centered on next-generation sequencing (NGS) technology. By designing multiplex PCR primers targeting conserved regions of the T cell receptor (TCR) gene, we specifically amplify gene fragments containing the Complementary Determining Region 3 (CDR3), which determines T cell specificity. CDR3 represents the most variable region within the TCR, functioning as a "molecular barcode" where its unique sequence defines a specific clonal type. By performing high-throughput sequencing of these CDR3 sequences and conducting bioinformatics quantitative analysis, we can precisely map the composition and dynamics of the entire T cell population within your sample.

How can we perform integrated analysis with multi-omics data?

The data formats we provide are compatible with mainstream transcriptomic and genomic analysis workflows. For example, you can combine TCR clonality data with RNA-seq data from the same sample to analyze the correlation between specific T cell clonality and immune checkpoint gene expression, cytokine profiles, and other factors within the tumor microenvironment. This enables a deeper, systems-level understanding of immune response mechanisms.

How do we distinguish meaningful clonal amplification from background noise in data analysis?

We employ a rigorous bioinformatics workflow: First, we remove amplification and sequencing errors; Second, we only recognize clonal types as genuine if they exceed a threshold percentage (e.g., 0.001%) of total sequencing reads. Finally, by aligning against experimental background controls or healthy donor data, we further filter out common sequencing background or non-specific amplification products, ensuring reported clonal amplifications possess biological significance.

Q: How do you ensure data reproducibility?

Our protocols include spike-in controls, duplicate sequencing, and cross-validation with orthogonal methods. We adhere to strict QC standards throughout the workflow.

Q: Can you handle longitudinal study designs?

Yes, we specialize in time-series analyses and paired sample comparisons, providing insights into clonal evolution and persistence.

Q: What bioinformatics approaches do you employ?

We use established tools (MiXCR, IMGT) complemented by custom algorithms for clonotype calling, diversity analysis, and visualization.

Q: How is data confidentiality maintained?

We implement encrypted storage, secure transfer protocols, and strict confidentiality agreements compliant with GDPR and HIPAA regulations.

References

Chen, Lei et al. "An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies." BMC immunology vol. 22,1 65. 28 Sep. 2021, https://doi.org/10.1186/s12865-021-00455-3
Distributed under Open Access license CC BY 4.0, without modification.

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.