Custom N-Linked Oligosaccharide Synthesis

N-linked oligosaccharides perform important roles in protein folding and stability, biosynthetic quality control, and sorting events in the secretory pathway of many N-glycosylated proteins. Equipped with world-leading technology platforms and professional scientific staff in carbohydrate synthesis, Creative Biolabs has accumulated extensive experience in custom N-linked oligosaccharides synthesis service. We offer our clients high-quality N-linked oligosaccharides from milligrams to grams scale in a short time with competitive pricing. Through our high-efficient system, clients could accelerate their research by reducing project costs and timelines.

The Role of N-Linked Oligosaccharides

Typified by the attachment of the glycan to an asparagine side chain of the protein, the N-linked glycosylation, is by far the most common. The consensus sequence for N-glycosylation is Asn-X-Ser/Thr, where X is any amino acid other than proline. The asparagine is linked to N-acetylglucosamine (GlcNAc) residues. Additional sugar residues in the glycan depend on whether glycosylation is high-mannose hybrid or complex type.

N-linked glycoproteins include such biologically important molecules as cell-surface receptors, cell-adhesion molecules, immunoglobulins, and other serum proteins, and tumor antigens. N-linked oligosaccharides perform important roles in those glycoprotein function. 1) N-linked oligosaccharides enhance the solubility and stability of the proteins in the endoplasmic reticulum, the Golgi and on the outside of the cell membrane. 2) N-linked oligosaccharides are used as signal molecules during protein folding and transport. 3) Most importantly, N-linked oligosaccharides on completely folded proteins are involved in a wide range of processes, including determining the specificity of membrane receptors in innate immunity or in cell-to-cell interactions, and changing the properties of hormones and secreted proteins. Statistical analyses show that instances of N-glycosylation in eukaryotic glycoproteins easily out-number O-glycosylation. In general, N-glycans are bulkier and more homogeneous in nature than O-glycans. The observations that so many eukaryotic proteins are glycosylated and that N-linked glycosylation is more frequently observed in proteins suggest that N-linked glycans are a prime requisite for maintaining the ‘fold–function’ balance in many proteins.

Fig.1 Schematic representation of N-glycosylation and O-glycosylation. (Mitra, 2006)

Custom N-Linked Oligosaccharide Synthesis at Creative Biolabs

Creative Biolabs focuses on research and development of carbohydrate synthetic strategies. Our scientists have established highly effective chemical or chemoenzymatic synthesis approaches for high-yield and cost-effective production of N-linked oligosaccharides. We have accumulated extensive experience in synthesizing complex and biomedically important N-linked oligosaccharides.

Highlights

• We have successfully synthesized many kinds of N-linked oligosaccharides.
• Chemical or chemoenzymatic synthesis.
• High flexibly, multi-step synthesis is available.
• Syntheses of N-linked oligosaccharides that you may require.
• We guarantee high-quality products.

In terms of the extensive experience in oligosaccharide synthesis, Creative Biolabs is proud to offer our clients custom N-linked oligosaccharides synthesis service with the best quality. Please contact us for more information and a detailed quote.

Reference

1. Mitra, N.; et al. N-linked oligosaccharides as outfitters for glycoprotein folding, form and function. Trends Biochem Sci. 2006, 31(3):156-63.

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