CytoFinal™ Low Sample Volume Mouse Lactate Dehydrogenase (LDH) ELISA Kit

CAT#: ITS-0326-WB110
Product Type: ELISA Kit
Species: Mouse
Target: Mouse lactate dehydrogenase
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitative ELISA kit for detecting mouse lactate dehydrogenase using minimal sample volumes.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Low Sample Volume Mouse Lactate Dehydrogenase (LDH) ELISA Kit enables sensitive quantification of mouse LDH in biological samples while requiring minimal input volume. Utilizing a high-performance sandwich ELISA format with HRP-based colorimetric detection, this kit provides reliable measurement of LDH levels in serum, plasma, cell culture supernatants, and tissue extracts for cytotoxicity and metabolic studies.
Features
Optimized sandwich ELISA configuration enables sensitive detection of mouse LDH while minimizing sample consumption in precious or limited-volume biological specimens.
High-affinity antibody pairs provide strong specificity and low background signals, ensuring accurate quantification across diverse experimental sample matrices.
HRP-TMB colorimetric detection generates stable signals compatible with standard microplate readers, facilitating efficient data acquisition and analysis.
Complete kit configuration including precoated microplates, calibration standards, buffers, and detection reagents supports reproducible workflows and simplified experimental setup.
Process Relevance
Quantifies LDH release to assess cellular membrane integrity, cytotoxicity, and metabolic stress in experimental systems.
Application Stage
Preclinical research, drug discovery, disease modeling, and biomarker investigation.
Applications
Measurement of mouse LDH levels in serum, plasma, cell culture supernatants, and tissue lysates for cytotoxicity assessment, metabolic research, inflammation studies, ADME profiling studies, and therapeutic response evaluation.
Target
Mouse lactate dehydrogenase
Detection Method
Colorimetric
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Cancer metabolism, immunology and inflammation, toxicology, cardiovascular research, metabolic biology, pharmacokinetics (ADME), cellular injury studies, etc.
Size
96 tests
Data Output Type
Quantitative colorimetric measurement of LDH concentration based on ELISA absorbance values.
Assay Range
0.156 ng/ml - 10 ng/ml
LOD
< 0.07 ng/ml
Species
Mouse
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lactate dehydrogenase (LDH) is a cytosolic enzyme broadly expressed in mammalian tissues and plays a central role in anaerobic energy metabolism. It catalyzes the reversible conversion of pyruvate and lactate while maintaining cellular NAD+/NADH balance. Because LDH is rapidly released following membrane disruption, extracellular LDH levels serve as a widely used indicator of cell damage and tissue injury.
Pathways
LDH participates in the terminal step of glycolysis by converting pyruvate to lactate while regenerating NAD+ required for sustained glycolytic activity. This reaction becomes particularly important under hypoxic conditions or during rapid cellular proliferation. Increased LDH activity is frequently associated with metabolic reprogramming pathways, including enhanced glycolysis observed in tumor cells and stressed tissues.
For Research Use Only | Not For Clinical Use
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