MetaVital™ Human Dimethylaniline monooxygenase [N-oxide-forming] 3 (FMO3) ELISA Kit

CAT#: ITS-0326-WB13
Product Type: ELISA Kit
Species: Human
Target: Human flavin-containing monooxygenase 3 (FMO3)
Brand: MetaVital™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies human FMO3 protein levels in biological samples using a sensitive sandwich ELISA assay.
Brand
MetaVital™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
MetaVital™ Human Dimethylaniline Monooxygenase [N-oxide-forming] 3 (FMO3) ELISA Kit provides a reliable platform for quantitative detection of FMO3 protein in human biological samples. Utilizing a highly specific sandwich ELISA format with optimized antibody pairs, the assay enables sensitive measurement of this important drug-metabolizing enzyme, supporting pharmacokinetic research, toxicology studies, and investigations of xenobiotic metabolism.
Features
Highly specific capture and detection antibodies enable selective measurement of human FMO3 with minimal cross-reactivity to related flavin-containing monooxygenase isoforms.
Sensitive sandwich ELISA detection allows accurate quantification of low-abundance enzyme levels across serum, plasma, tissue lysates, and cultured cell extracts.
Standard microplate assay format supports reproducible measurements and convenient integration into pharmacological and metabolic research workflows.
Carefully optimized reagents and standardized protocol ensure consistent assay performance across multiple biological sample types and experimental conditions.
Process Relevance
Quantifies FMO3 expression to assess metabolic enzyme activity involved in xenobiotic detoxification and drug metabolism.
Application Stage
Drug metabolism research, pharmacokinetic studies, toxicology investigations, and biomarker analysis.
Applications
Quantitative detection of FMO3 protein in serum, plasma, tissue lysates, or cell extracts for metabolic enzyme profiling, pharmacological studies, ADME profiling studies, and xenobiotic metabolism research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Human flavin-containing monooxygenase 3 (FMO3)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Drug metabolism & pharmacokinetics (ADME), toxicology, pharmacology, enzymology, biomarker research, xenobiotic metabolism, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using colorimetric sandwich ELISA detection.
Assay Range
0.156 ng/ml - 10 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Flavin-containing monooxygenase 3 (FMO3) is a major hepatic Phase I metabolic enzyme responsible for oxidizing nitrogen-, sulfur-, and phosphorus-containing compounds. It plays a critical role in the detoxification of drugs, environmental chemicals, and dietary metabolites. Variations in FMO3 expression or function can influence metabolic clearance and contribute to metabolic disorders such as trimethylaminuria.
Pathways
FMO3 participates in the Phase I xenobiotic metabolism pathway, catalyzing NADPH-dependent oxygenation reactions that convert lipophilic substrates into more polar metabolites. These reactions often complement cytochrome P450-mediated oxidation. The resulting metabolites are subsequently processed by Phase II conjugation enzymes, including sulfotransferases and UDP-glucuronosyltransferases, facilitating efficient detoxification and elimination.
For Research Use Only | Not For Clinical Use
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