ConjuLink™ Human Tissue-specific SULT1A1 / Sulfotransferase 1A1 ELISA Kit

CAT#: ITS-0326-WB11
Product Type: ELISA Kit
Species: Human
Target: Human sulfotransferase 1A1 (SULT1A1)
Brand: ConjuLink™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies human SULT1A1 protein levels in tissue-derived samples using a sensitive sandwich ELISA assay.
Brand
ConjuLink™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
ConjuLink™ Human Tissue-specific SULT1A1 / Sulfotransferase 1A1 ELISA Kit enables accurate quantification of SULT1A1 protein expression in human tissue lysates. Utilizing a highly specific sandwich ELISA format with validated antibody pairs, this assay supports sensitive detection of sulfotransferase 1A1 across diverse tissue samples, facilitating metabolic enzyme profiling, toxicological studies, and research on xenobiotic detoxification pathways.
Features
Highly selective antibody pairs ensure specific detection of human SULT1A1, minimizing cross-reactivity with related sulfotransferase isoforms in complex tissue samples.
Sensitive sandwich ELISA platform enables accurate quantification of low-abundance enzyme expression within tissue lysates and biological extracts.
Standard microplate-based assay workflow ensures consistent performance and straightforward integration into routine enzymology and pharmacological research pipelines.
Carefully optimized reagents and detection chemistry support reproducible measurements across multiple tissue types and experimental conditions.
Process Relevance
Quantifies tissue-specific SULT1A1 expression to evaluate metabolic capacity and xenobiotic detoxification pathways.
Application Stage
Pharmacology research, toxicology studies, metabolic enzyme profiling, and biomarker investigation.
Applications
Quantitative analysis of SULT1A1 protein expression in human tissue lysates, metabolic enzyme profiling, biomarker discovery, pharmacological studies, ADME profiling studies, and investigations of sulfation-mediated detoxification mechanisms.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Human sulfotransferase 1A1 (SULT1A1)
Detection Method
Colorimetric - 450nm (TMB)
Sample Type
Cell lysates, tissue homogenates
Assay Measures
Quantitative
Research Areas
Drug metabolism & pharmacokinetics (ADME), toxicology, pharmacology, enzymology, biomarker discovery, xenobiotic metabolism, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using colorimetric sandwich ELISA detection.
Assay Range
0.156 - 10 ng/ml
LOD
0.057 ng/ml
Species
Human
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Sulfotransferase 1A1 (SULT1A1) is a cytosolic Phase II metabolic enzyme widely expressed in human tissues such as liver and intestine. It catalyzes sulfation reactions that modify phenolic compounds, hormones, and xenobiotics. These modifications increase molecular polarity, enabling efficient detoxification and elimination of biologically active molecules.
Pathways
SULT1A1 functions in the Phase II sulfation pathway of xenobiotic metabolism, catalyzing sulfate transfer from the cofactor 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to target substrates. This conjugation step often follows oxidative metabolism mediated by cytochrome P450 enzymes. The coordinated activity of Phase I and Phase II pathways regulates detoxification, hormone metabolism, and drug clearance.
For Research Use Only | Not For Clinical Use
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