CytoFinal™ Pig Aspartate Aminotransferase, Mitochondrial / AST (GOT2) ELISA Kit

CAT#: ITS-0326-WB102
Product Type: ELISA Kit
Species: Pig
Target: Mitochondrial aspartate aminotransferase (AST/GOT2)
Brand: CytoFinal™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantifies mitochondrial AST (GOT2) levels in pig tissues, cell lysates, and biological fluids using a sensitive ELISA method.
Brand
CytoFinal™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
CytoFinal™ Pig AST (GOT2) ELISA Kit provides an in vitro solution for precise quantification of mitochondrial aspartate aminotransferase in pig-derived samples. Optimized for tissue homogenates, cell lysates, and biological fluids, it ensures high accuracy, reproducibility, and user-friendly operation for research and diagnostic applications.
Features
High sensitivity detection with a dynamic range of 0.78-50 ng/mL, enabling precise quantification of low-abundance GOT2.
Specific sandwich ELISA format pre-coated with validated antibodies, minimizing cross-reactivity and ensuring robust results.
Colorimetric readout using TMB substrate for straightforward measurement at 450 nm, compatible with standard microplate readers.
Complete kit includes lyophilized standards, HRP-conjugated reagents, wash buffers, and detailed protocols for consistent assay performance.
Process Relevance
Monitoring mitochondrial AST (GOT2) levels to support metabolic and cellular function studies in pig models.
Application Stage
Drug discovery, toxicology studies, metabolic research, and in-process quality assessment.
Applications
Suitable for quantitative ELISA in tissue homogenates, cell lysates, serum, or plasma; ideal for metabolic profiling, mitochondrial activity studies, ADME profiling studies, and biomarker analysis.
Target
Mitochondrial aspartate aminotransferase (AST/GOT2)
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Metabolic research, toxicology, pharmacokinetics (ADME), veterinary medicine, mitochondrial physiology, biomarker discovery, cellular metabolism, enzyme kinetics, nutritional studies, etc.
Size
96 tests
Data Output Type
Quantitative enzyme concentration measurement via colorimetric sandwich ELISA.
Assay Range
0.78 ng/ml - 50 ng/ml
Species
Pig
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
GOT2 is a mitochondrial enzyme essential for amino acid metabolism and the malate-aspartate shuttle, supporting energy production. Its activity reflects cellular metabolic status, tissue integrity, and mitochondrial function in pigs, providing a translational model for human metabolic studies.
Pathways
GOT2 participates in the malate-aspartate shuttle, linking cytosolic and mitochondrial NADH pools. Upstream substrates include glutamate and oxaloacetate, while downstream products feed into the TCA cycle and gluconeogenesis, influencing energy metabolism.
For Research Use Only | Not For Clinical Use
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