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45-1 Ramsey Road, Shirley, NY 11967, USA
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Anti-CA9 CAR-T Preclinical in vivo Assay

Target Background

CA9/CAIX (carbonic anhydrase 9) is a zinc metalloenzyme catalyzing the reversible hydration of carbon dioxide. Physiologically, CA9 participates in a variety of biological processes including respiration, calcification, acid-based balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, gastric acid, etc. as a transmembrane protein, and involves in cell proliferation and transformation. With persistent efforts, researchers have revealed that CA9 expression levels associate with various clinicopathological outcomes. For instance, CA9 is now regarded as a cellular biomarker of hypoxia. Furthermore, it is expressed in all clear-cell renal cell carcinoma (ccRCC) and hypoxic solid tumors as one of the only two known tumor-associated carbonic anhydrase isoenzymes, while undetectable in normal kidney and most other normal tissues. In addition, Girentuximab, an anti-CA9 monoclonal Ab, has been investigated in clinical trials for RCC. Creative Biolabs is able to help researchers to initiate the very first anti-CA9 CAR-T cell therapy.

CA9

A mouse with a subcutaneous CA9-positive ccRCC and proposed structural model of CA9 dimer on the cellular membrane
Proceedings of the National Academy of Sciences 106.38 (2009): 16233-16238
European urology 58.1 (2010): 75-83

Anti-CA9 CAR-T Cell Therapy

CA9 has been targeted with multiple monoclonal Abs in clinical trials for the treatment of RCC which have resulted in limited efficiency, and the pilot pre-clinical and clinical CAR-T cell therapy has not yet been reported. Creative Biolabs helps researchers fill in the blank for the cutting-edge immunotherapy.

Animal Models for in vivo Study of anti-CA9 CAR-T Cell Therapy

Creative Biolabs offers researchers almost every particular animal model in the world via the major techniques including the conventional carcinogen-induced models, the mosaic transposon- or virus-induced models, the transgenic models and the subcutaneous or patient-derived xenograft models, and the talented creation of specific requirement to make a stand against RCC.

Mouse Kidney

Mouse Kidney
Development143.4 (2016): 595-608

In vivo Assay Parameters and Techniques

At Creative Biolabs, we offer the most exquisite and comprehensive service platform for preclinical anti-CA9 CAT-T cell therapy research.
Efficacy Test
Tumor remission monitored by tumor volume recording or bioluminescence imaging and survival curve tracking
Viability and Bio-distribution Studies
Durability, GLP-compliant bio-distribution studies
Toxicity Evaluation
Pilot tolerability (MTD, The route of administration, Dose regimen/response/onset)
Clinical observation (body weight, feed consumption, ophthalmologic and clinical pathology)
Cytokine storm surveillance (fever, hypertension, prolonged cytopenia)
Complete necropsy, organ weight
Histopathology
Tumorigenicity study

You request, Creative Biolabs offers the best.

References

  1. Alterio, Vincenzo, et al. "Crystal structure of the catalytic domain of the tumor-associated human carbonic anhydrase IX." Proceedings of the National Academy of Sciences 106.38 (2009): 16233-16238.
  2. Stillebroer, Alexander B., et al. "Carbonic anhydrase IX in renal cell carcinoma: implications for prognosis, diagnosis, and therapy." European urology 58.1 (2010): 75-83.
  3. O'Brien, Lori L., et al. "Differential regulation of mouse and human nephron progenitors by the Six family of transcriptional regulators." Development143.4 (2016): 595-608.

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CONTACT US

USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-871-5806
Fax: 1-631-207-8356
Email:

Europe
Ringstrasse 4, 64401 Gross-Bieberau, Germany
Tel: 44-207-048-3343

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