CellRapeutics™ BrdU Staining Kit(CART-026CL)
CellRapeutics™ BrdU Staining Kit
The BrdU Staining Kit for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cells of mammalian species by flow cytometric analysis. Cycling cells are incubated with 5-bromo-2'-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, the cells are stained for BrdU incorporation along with any other cell surface and/or intracellular targets of interest.
This kit is optimized to achieve brighter staining compared to traditional protocols.
BrdU: 5 x 1 mL sterile vials; store at less than or equal to -70°C.
DNase I: 10 x 0.3 mL vials; store at less than or equal to -70°C.
Anti-BrdU APC Antibody (clone BU20A): 1 x 100 test vial; store at 2-8°C.
BrdU Staining Buffer Concentrate (4X): 1 x 30 mL bottle; store at 2-8°C.
Fixation/Permeabilization Diluent: 1 x 100 mL bottle; store at 2-8°C.
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis Applications Tested The BrdU Staining Kit for Flow Cytometry APC has been tested by flow cytometric analysis of BrdU-labeled mouse
splenocytes using the attached protocol. The BU20A antibody can be used at 5 μL (0.125 μg) per test. A test is
defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
Bovine, Canine, Cow, Human, Monkey, Mouse, Rat, Rhesus
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.