CellRapeutics™ BrdU Staining Kit(CART-026CL)


Product Name
CellRapeutics™ BrdU Staining Kit
Cat. No.
CART-026CL
General Description
The BrdU Staining Kit for Flow Cytometry contains the necessary reagents and buffers for identifying and examining proliferating cells of mammalian species by flow cytometric analysis. Cycling cells are incubated with 5-bromo-2'-deoxyuridine (BrdU), a synthetic analog of thymidine which incorporates into newly synthesized genomic DNA during the S-phase of mitosis. Following DNA denaturation, the cells are stained for BrdU incorporation along with any other cell surface and/or intracellular targets of interest.

This kit is optimized to achieve brighter staining compared to traditional protocols.
Components
BrdU: 5 x 1 mL sterile vials; store at less than or equal to -70°C.
DNase I: 10 x 0.3 mL vials; store at less than or equal to -70°C.
Anti-BrdU APC Antibody (clone BU20A): 1 x 100 test vial; store at 2-8°C.
BrdU Staining Buffer Concentrate (4X): 1 x 30 mL bottle; store at 2-8°C.
Fixation/Permeabilization Diluent: 1 x 100 mL bottle; store at 2-8°C.
Laser
Red Laser
Emit
660 nm
Excite
633 - 647 nm
Applications
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis Applications Tested The BrdU Staining Kit for Flow Cytometry APC has been tested by flow cytometric analysis of BrdU-labeled mouse
splenocytes using the attached protocol. The BU20A antibody can be used at 5 μL (0.125 μg) per test. A test is
defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
Species Reactivity
Bovine, Canine, Cow, Human, Monkey, Mouse, Rat, Rhesus

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Related Products


Kits

Online Inquiry

Name:
* Phone:
*E-mail Address:
*Service & Products Interested:
Project Description:
*Verification Code:
Please input "biolabs"(case insensitive) as verification code.
CB Promise
CONTACT US
45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-871-5806
Europe: 44-207-097-1828
Fax: 1-631-207-8356
Email:
CONTACT US

USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-871-5806
Fax: 1-631-207-8356
Email:

Europe
Ringstrasse 4, 64401 Gross-Bieberau, Germany
Tel: 44-207-097-1828

Distributors

To view the contact information for a specific location, select the desired country:

Australia
australia

FOLLOW US ON