Creative Biolabs has a tradition of commitment. To achieve efficient execution and regulatory approval, we offer careful considerations of your program for the development of a cellular or gene therapy product – now and in the future.
EXPLORE MORE HighlightsWe focus on unmet needs and develop novel cellular and gene drugs and solutions that offer significant benefits over existing options.
EXPLORE MORE HighlightsThe advent of Chimeric Antigen Receptor T-cell (CAR-T) therapies has revolutionized the field of oncology, providing new avenues for treating various forms of cancer. Our 20 years of experience in the biotechnology sector have equipped us with the expertise and technological capabilities to support the entire lifecycle of CAR-T products, from early development to commercialization.
EXPLORE MORETo accelerate advanced breakthroughs of your projects, we offer broad range of platforms which enable our clients be free to tackle problems with cutting-edge technologies from different angles and in different methods.
EXPLORE MORE HighlightsUse the resources in our library to help you understand your options and make critical decisions for your study. We offer oncolytic virus, CAR-T, and dendritic cell related documents, as well as newsletter. If you don't find the answers you're looking for, contact us for additional assistance.
EXPLORE MORE HighlightsGet a real taste and understanding of the business and culture of one of the world's great research-based cellular and gene therapy discovery and development companies.
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Creative Biolabs offers optimized electroporation gene transfer service for modifying T cells genetically. As a non-viral gene transfer system, electroporation performs high transfection efficiency and low toxicity compared with other non-viral gene transfer methods used for primary lymphocytes, such as liposome. It can also accelerate gene transfer without the potential safety issues from viral vectors.
There are two classes of methods for DNA transfer: natural methods and artificial methods (including physical methods, chemical methods and electrical methods). Electroporation, along with electrofusion, belong to electrical methods. Electrical methods share similar mechanism: the reversible electropermeabilization of biological membranes. Electroporation employs high-voltage electrical pulse to introduce agents into cells. Just as shown in the figure, E-field (electric field) is used to introduce microscopic pores in biological membranes. Then these microscopic pores, electropores, allow different agents such as small molecules, ions to pass from one side of the membrane to the other. When a suitable pulse is given, the pores can reseal with molecules inside and the cells recover. Compare with some other gene transfer strategies, the approach of electroporation has several advantages: its process is fast and less costly. It is applied for a number of cell types and can treat various cells simultaneously. Moreover, by this method, high percentage of stable transformants can be produced. Considering these advantages, electroporation has various applications including: (1) to introduce exogenous DNA into bacterial protoplast, yeast protoplast, plant protoplast and animal cell line; (2) to increase efficiency of transfection or transformation of different cells; (3) to transform plant cells including wheat, tobacco, rice, maize with frequency up to 1%; (4) to introduce DNA into early embryo to produce transgenic animals; (5) to generate transient expression of molecular constructs. In terms of mammalian transgenesis, electroporation has been used to transfer exogenous DNA into cultured mammalian embryos at defined stages of development. Similarly, bovine blastocysts and fish species can also be genetically manipulated by the method of electroporation. Besides to facilitate gene transfer in both large and small animals, electroporation is reported to increase gene expression and improve immune response elicited to DNA vaccines.
The electroporation methods include:
The most popular strategies used for TCR gene transfer:
| ➤ | Viral vectors: |
Retroviral vectors Lentiviral vectors |
| ➤ | Electroporation |
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All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.
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