Fluorescence Resonance Energy Transfer (FRET) Assay

All products and services are For Research Use Only and CANNOT be used in the treatment or diagnosis of disease.

Creative Biolabs has generated chimeric antigen receptors (CARs) specific for a variety of target antigens, many of which are overexpressed on tumor cells. Based on experience in CAR-T, we are pioneering the development of chimeric antigen receptor macrophage (CAR-MA). Moreover, we offer high-quality custom fluorescence resonance energy transfer (FRET) assay.

Introduction

FRET allows the detection of the approach between two molecules within several nanometers. The mechanism of FRET involves a donor fluorophore in an excited electronic state, which may transfer its excitation energy to a nearby acceptor chromophore in a non-radiative fashion through long-range dipole-dipole interactions. FRET can be used to measure the distance between the donor and the acceptor or the dynamical activities between two sites on this macromolecule, such as protein interactions. This technique is widely applied in many fields such as single-molecule experiments, molecular motors, biosensors, and DNA mechanical movements. FRET is extremely useful for biomedical research for three major reasons:

Due to its exquisite sensitivity to molecular distance, FRET makes a great reporter for proximity as well as a very small perturbation in proximity.
FRET is particularly suitable for biomedical research is that the distance range at which FRET occurs matches well with the dimension of many biological molecules.
FRET has the advantage of being applicable in living biological systems under physiological conditions and, in many cases, in real-time.

Fig.1 The mechanism of FRET. (Wu, 2020)

Applications

In fluorescence microscopy, fluorescence confocal laser scanning microscopy, as well as in molecular biology, is a useful tool to quantify molecular dynamics in biophysics and biochemistry, such as protein-protein interactions, protein-DNA interactions, and protein conformational changes. FRET can also be used in techniques such as flow cytometry, immunocytochemistry, immunohistochemistry, and ELISA, to identify potential molecular interactions.

Biological membrane mapping: interaction of proteins that is present on the surface of the cell.
Monitor and locate ion binding and protein-protein interactions in living cells.
Probing protein conformational changes.
FRET-based biosensors.
DNA hybridization detection.
DNA mutation detection.

Creative Biolabs provides custom FRET analysis for function research such as protein-protein interaction. If you are interested in our services, please feel free to contact us.

Reference

Wu, L.; et al. Förster resonance energy transfer (FRET)-based small-molecule sensors and imaging agents. Chemical Society Reviews. 2020, 49(15): 5110-5139.

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